Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON M5S 1A8, Canada.
Krembil Brain Institute, Toronto Western Hospital, University Health Network, Toronto, ON M5T 2S8, Canada.
Int J Mol Sci. 2024 Jun 7;25(12):6335. doi: 10.3390/ijms25126335.
The NLRP3 inflammasome plays a crucial role in the inflammatory response, reacting to pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs). This response is essential for combating infections and restoring tissue homeostasis. However, chronic activation can lead to detrimental effects, particularly in neuropsychiatric and neurodegenerative diseases. Our study seeks to provide a method to effectively measure the NLRP3 inflammasome's activation within cerebral organoids (COs), providing insights into the underlying pathophysiology of these conditions and enabling future studies to investigate the development of targeted therapies.
NLRP3 炎性小体在炎症反应中起着至关重要的作用，它可以对病原体相关分子模式（PAMPs）和损伤相关分子模式（DAMPs）做出反应。这种反应对于对抗感染和恢复组织内稳态是必不可少的。然而，慢性激活会导致有害的影响，特别是在神经精神和神经退行性疾病中。我们的研究旨在提供一种在大脑类器官（COs）中有效测量 NLRP3 炎性小体激活的方法，深入了解这些疾病的潜在病理生理学，并为未来的研究提供靶向治疗的发展方向。
