Tian Si-Qin, Cui Ping, Fang Su-Fang, Liu Guo-Hua, Wang Chun-Ren, Zhu Xing-Quan
a College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University , Daqing , Heilongjiang Province , P. R. China .
b State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province , Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Lanzhou , Gansu Province , P. R. China .
Mitochondrial DNA. 2015;26(5):714-5. doi: 10.3109/19401736.2013.843088. Epub 2013 Dec 11.
In the present study, we determined the complete mitochondrial DNA (mtDNA) sequence of Eimeria magna from rabbits for the first time, and compared its gene contents and genome organizations with that of seven Eimeria spp. from domestic chickens. The size of the complete mt genome sequence of E. magna is 6249 bp, which consists of 3 protein-coding genes (cytb, cox1 and cox3), 12 gene fragments for the large subunit (LSU) rRNA, and 7 gene fragments for the small subunit (SSU) rRNA, without transfer RNA genes, in accordance with that of Eimeria spp. from chickens. The putative direction of translation for three genes (cytb, cox1 and cox3) was the same as those of Eimeria species from domestic chickens. The content of A + T is 65.16% for E. magna mt genome (29.73% A, 35.43% T, 17.09 G and 17.75% C). The E. magna mt genome sequence provides novel mtDNA markers for studying the molecular epidemiology and population genetics of Eimeria spp. and has implications for the molecular diagnosis and control of rabbit coccidiosis.
在本研究中,我们首次测定了兔巨型艾美耳球虫的完整线粒体DNA(mtDNA)序列,并将其基因内容和基因组组织与来自家鸡的七种艾美耳球虫进行了比较。巨型艾美耳球虫完整线粒体基因组序列的大小为6249 bp,由3个蛋白质编码基因(细胞色素b、细胞色素c氧化酶亚基1和细胞色素c氧化酶亚基3)、12个大核糖体亚基(LSU)rRNA基因片段和7个小核糖体亚基(SSU)rRNA基因片段组成,没有转运RNA基因,这与来自鸡的艾美耳球虫一致。三个基因(细胞色素b、细胞色素c氧化酶亚基1和细胞色素c氧化酶亚基3)的推定翻译方向与家鸡艾美耳球虫的相同。巨型艾美耳球虫线粒体基因组的A + T含量为65.16%(A为29.73%,T为35.43%,G为17.09%,C为17.75%)。巨型艾美耳球虫线粒体基因组序列为研究艾美耳球虫的分子流行病学和群体遗传学提供了新的mtDNA标记,对兔球虫病的分子诊断和防控具有重要意义。