可微型化的化学发光系统，用于水中的 ATP 检测。

Miniaturizable Chemiluminescence System for ATP Detection in Water.

机构信息

Istituto per la Microeletttronica e Microsistemi-Consiglio Nazionale delle Ricerche, VIII Strada Z.I., 5, 95121 Catania, Italy.

Department of Chemical Sciences, University of Catania, Viale Andrea Doria 6, 95125 Catania, Italy.

出版信息

Sensors (Basel). 2024 Jun 17;24(12):3921. doi: 10.3390/s24123921.

DOI:10.3390/s24123921

PMID:38931704

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11207618/

Abstract

We present the design, fabrication, and testing of a low-cost, miniaturized detection system that utilizes chemiluminescence to measure the presence of adenosine triphosphate (ATP), the energy unit in biological systems, in water samples. The ATP-luciferin chemiluminescent solution was faced to a silicon photomultiplier (SiPM) for highly sensitive real-time detection. This system can detect ATP concentrations as low as 0.2 nM, with a sensitivity of 79.5 A/M. Additionally, it offers rapid response times and can measure the characteristic time required for reactant diffusion and mixing within the reaction volume, determined to be 0.3 ± 0.1 s. This corresponds to a diffusion velocity of approximately 44 ± 14 mm/s.

摘要

我们提出了一种低成本、微型化的检测系统的设计、制造和测试，该系统利用化学发光来测量水样中生物系统中的能量单位三磷酸腺苷（ATP）的存在。ATP-荧光素化学发光溶液面对硅光电倍增管（SiPM）进行高灵敏度实时检测。该系统可以检测低至 0.2 nM 的 ATP 浓度，灵敏度为 79.5 A/M。此外，它还具有快速的响应时间，可以测量反应体积内反应物扩散和混合所需的特征时间，确定为 0.3 ± 0.1 s。这对应于大约 44 ± 14 mm/s 的扩散速度。