Zhu Xuedong, Wen Shiqi, Gul Hameed, Xu Pan, Yang Yang, Liao Ximei, Ye Yunling, Xu Zijian, Zhang Xiaofang, Wu Lin
Fuling Academy of Southwest University/Southeast Chongqing Academy of Agricultural Sciences, Southwest University, Chongqing, China.
Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Southwest University, Chongqing, China.
Front Plant Sci. 2024 Jun 12;15:1409601. doi: 10.3389/fpls.2024.1409601. eCollection 2024.
leaves are highly valued in traditional Chinese medicine for their substantial concentration of flavonoids, which play a crucial role in manifesting the plant's therapeutic properties. This study investigated the metabolomic, transcriptomic and proteomic profiles of leaves from two cultivars, (J) and (R), at three different developmental stages. Metabolite identification and analysis revealed a total of 1,412 and 1,421 metabolites with known structures were found. Flavonoids made up of 33%, including 10 significant accumulated icariin analogues. Transcriptomic analysis unveiled totally 41,644 differentially expressed genes (DEGs) containing five encoded genes participated in icariin biosynthesis pathways. Totally, 9,745 differentially expressed proteins (DEPs) were found, including Cluster-47248.2.p1 (UDP-glucuronosy/UDP-glucosyltransferase), Cluster-30441.2.p1 (O-glucosyltransferase), and Cluster-28344.9.p1 (anthocyanidin 3-O-glucoside 2 "-O-glucosyltransferase-like) through proteomics analysis which are involved to icariin biosynthesis. Protein-protein interaction (PPI) assay exhibited, totally 12 proteins showing a strong relationship of false discovery rate (FDR) <0.05 with these three proteins containing 2 leucine-rich repeat receptor kinase-like protein SRF7, and 5 methyl jasmonate esterase 1. Multi-omics connection networks uncovered 237 DEGs and 72 DEPs exhibited significant associations with the 10 icariin analogues. Overall, our integrated omics approach provides comprehensive insights into the regulatory network underlying icariin synthesis in , offering valuable resources for further research and development in medicinal plant cultivation and pharmaceutical applications.
叶子在传统中药中具有很高的价值，因为它们含有大量的黄酮类化合物，这些黄酮类化合物在展现植物的治疗特性方面起着至关重要的作用。本研究调查了两个品种（J）和（R）在三个不同发育阶段的叶子的代谢组学、转录组学和蛋白质组学特征。代谢物鉴定和分析发现，共有1412种和1421种具有已知结构的代谢物。黄酮类化合物占33%，其中包括10种显著积累的淫羊藿苷类似物。转录组分析共发现41644个差异表达基因（DEG），其中有5个编码基因参与淫羊藿苷生物合成途径。通过蛋白质组学分析，共发现9745个差异表达蛋白（DEP），包括Cluster-47248.2.p1（UDP-葡萄糖醛酸基/UDP-葡萄糖基转移酶）、Cluster-30441.2.p1（O-葡萄糖基转移酶）和Cluster-28344.9.p1（花青素3-O-葡萄糖苷2”-O-葡萄糖基转移酶样），它们都参与淫羊藿苷的生物合成。蛋白质-蛋白质相互作用（PPI）分析显示，共有12种蛋白质与这三种蛋白质的错误发现率（FDR）<0.05有很强的关系，其中包括2种富含亮氨酸重复序列的受体激酶样蛋白SRF7和5种茉莉酸甲酯酯酶1。多组学连接网络发现，237个DEG和72个DEP与10种淫羊藿苷类似物有显著关联。总体而言，我们的综合组学方法为淫羊藿苷合成的调控网络提供了全面的见解，为药用植物栽培和制药应用的进一步研究和开发提供了有价值的资源。
