Song Min, Linghu Bin, Huang Shuhua, Li Fang, An Ran, Xie Changgen, Zhu Yantao, Hu Shengwu, Mu Jianxin, Zhang Yanfeng
Hybrid Rapeseed Research Center of Shaanxi Province, Yangling, China.
State Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Yangling, China.
Front Plant Sci. 2022 Apr 12;13:865132. doi: 10.3389/fpls.2022.865132. eCollection 2022.
The leucine-rich repeat receptor-like protein kinase () family represents the largest group of RLKs in plants and plays vital roles in plant growth, development and the responses to environmental stress. Although families have been identified in many species, they have not yet been reported in . In this study, a total of 444 genes were identified in the genome of cultivar "Zhongshuang 11" (ZS11), and classified into 22 subfamilies based on phylogenetic relationships and genome-wide analyses. Conserved motifs and gene structures were shared within but not between subfamilies. The 444 genes were asymmetrically distributed on 19 chromosomes and exhibited specific expression profiles in different tissues and in response to stress. We identified six homologs and obtained partial knockouts via CRISPR/Cas9 technology, generating semi-dwarf lines without decreased yield compared with controls. This study provides comprehensive insight of the family in . Additionally, the semi-dwarf lines expand the "ideotype" germplasm resources and accelerate the breeding process for .
富含亮氨酸重复序列的类受体蛋白激酶（）家族是植物中最大的一类类受体蛋白激酶，在植物生长、发育及对环境胁迫的响应中发挥着至关重要的作用。尽管在许多物种中都已鉴定出家族，但在中尚未见报道。本研究在甘蓝型油菜品种“中双11号”（ZS11）基因组中总共鉴定出444个基因，并基于系统发育关系和全基因组分析将其分为22个亚家族。保守基序和基因结构在亚家族内部而非亚家族之间共享。这444个基因不对称地分布在19条染色体上，并在不同组织及响应胁迫时呈现出特定的表达谱。我们鉴定出6个同源基因，并通过CRISPR/Cas9技术获得了部分敲除植株，培育出了与对照相比产量未降低的半矮化株系。本研究全面深入地了解了甘蓝型油菜中的家族。此外，这些半矮化株系拓展了“理想株型”种质资源，并加速了甘蓝型油菜的育种进程。
