Lee K, Cargill C, Atkinson H
Aust Vet J. 1985 Mar;62(3):91-3. doi: 10.1111/j.1751-0813.1985.tb14147.x.
A simple enzyme linked immunosorbent assay (ELISA) was developed for the serological diagnosis of Brucella ovis infections in rams. Serums from brucellosis accredited-free flocks and flocks known to be infected with B. ovis were tested and the results correlated with warm complement fixation (CF) test and bacteriological examination of semen. Both the ELISA and the CF test detected 0.5% false positive reactions in rams from clinically negative flocks. However the ELISA detected significantly more positive reactors in infected flocks and the CF test failed to detect some rams excreting B. ovis. The ELISA proved to be a valuable test in eradicating brucellosis from infected flocks.
开发了一种简单的酶联免疫吸附测定（ELISA）法用于公羊布鲁氏菌感染的血清学诊断。对来自无布鲁氏菌病认证羊群和已知感染绵羊布鲁氏菌的羊群的血清进行了检测，并将结果与热补体结合（CF）试验以及精液的细菌学检查结果进行了关联。ELISA和CF试验在临床阴性羊群的公羊中均检测到0.5%的假阳性反应。然而，ELISA在感染羊群中检测到的阳性反应动物明显更多，而CF试验未能检测到一些排出绵羊布鲁氏菌的公羊。ELISA被证明是从感染羊群中根除布鲁氏菌病的一项有价值的检测方法。
