一种与慢性血液透析相关的新型淀粉样蛋白被鉴定为β2-微球蛋白。

A new form of amyloid protein associated with chronic hemodialysis was identified as beta 2-microglobulin.

作者信息

Gejyo F, Yamada T, Odani S, Nakagawa Y, Arakawa M, Kunitomo T, Kataoka H, Suzuki M, Hirasawa Y, Shirahama T

出版信息

Biochem Biophys Res Commun. 1985 Jun 28;129(3):701-6. doi: 10.1016/0006-291x(85)91948-5.

DOI:10.1016/0006-291x(85)91948-5

Abstract

Amyloid fibrils were isolated from amyloid-laden tissue obtained from a chronic hemodialysis patient with carpal tunnel syndrome. After solubilization in guanidine HCl, a significant amount of the protein was located in a homogeneous low molecular weight fraction. The protein was found to be identical to beta 2-microglobulin, with regard to its molecular weight of 11,000, amino acid composition and 16 amino-terminal amino acids: Ile-Gln-Arg-Thr-Pro-Lys-Ile-Gln-Val-Tyr-Ser-Arg-His-Pro-Ala-Glu-. These results demonstrate that the amyloid associated with chronic hemodialysis contains as major component a new form of amyloid fibril protein that is homologous to beta 2-microglobulin.

摘要

淀粉样纤维是从一名患有腕管综合征的慢性血液透析患者的富含淀粉样物质的组织中分离出来的。在盐酸胍中溶解后，大量蛋白质存在于一个均匀的低分子量组分中。就其11,000的分子量、氨基酸组成以及16个氨基末端氨基酸（异亮氨酸-谷氨酰胺-精氨酸-苏氨酸-脯氨酸-赖氨酸-异亮氨酸-谷氨酰胺-缬氨酸-酪氨酸-丝氨酸-精氨酸-组氨酸-脯氨酸-丙氨酸-谷氨酸）而言，该蛋白质被发现与β2-微球蛋白相同。这些结果表明，与慢性血液透析相关的淀粉样物质含有一种与β2-微球蛋白同源的新型淀粉样纤维蛋白作为主要成分。