Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Bio-Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, P. R. China.
Analyst. 2023 Feb 13;148(4):856-862. doi: 10.1039/d2an02045a.
Hydrazine (NH) produced by industries is distributed into different environments, and seriously threatens ecology and human security. Hence, it is important to develop probes that detect NH in various environments. In this study, a novel NH fluorescent probe was prepared based on a dibenzothiazole derivative (DBTD). The obtained DBTD probe demonstrated a strong ratio of colorimetric detection of NH, a rapid response, and good selectivity and sensitivity (detection limit 0.438 μM). Based on its good performance, the DBTD probe was successfully applied for the determination of trace NH in water, cells, and zebrafish. In addition, the results of the fluorescence colocalization experiment demonstrated the lysosomal-targetable ability of DBTD.
肼（NH）由工业生产产生并分布于不同环境中，严重威胁着生态和人类安全。因此，开发用于检测各种环境中 NH 的探针十分重要。本研究基于二苯并噻唑衍生物（DBTD）制备了一种新型 NH 荧光探针。所得到的 DBTD 探针对 NH 具有强烈的比色检测响应，响应迅速，选择性和灵敏度高（检测限 0.438 μM）。基于其良好的性能，DBTD 探针成功应用于水样、细胞和斑马鱼中痕量 NH 的测定。此外，荧光共定位实验的结果表明了 DBTD 具有溶酶体靶向能力。
