Machado Camila Dias, Farago Paulo Vitor, Costa Caroline de Moura, Farias Katyuce Souza, Silva Denise Brentan, Marques Aline Aparecida Macedo, Moreno Karyne Garcia Tafarelo, Pael Luana Ale Bertoncelo, da Silva Maria Luiza Fidelis, Gasparotto Junior Arquimedes, Manfron Jane
Pharmaceutical Sciences Post Graduate Program, State University of Ponta Grossa, Ponta Grossa, PR, Brazil.
Laboratory of Natural Products and Mass Spectrometry (LaPNEM), Faculty of Pharmaceutical Sciences, Food, and Nutrition (FACFAN), Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS, Brazil.
J Ethnopharmacol. 2024 Oct 28;333:118499. doi: 10.1016/j.jep.2024.118499. Epub 2024 Jun 25.
Schinus molle L. is a medicinal species belonging to the Anacardiaceae family. It is commonly referred to as "aroeira" and its leaves and roots are utilized for treating different pathological conditions. However, despite its widespread use in traditional medicine, there is a lack of in-depth toxicological studies.
To evaluate the acute toxicity and genotoxicity of S. molle aqueous extract/ethanol-soluble fraction in rats.
First, a purified aqueous extract was obtained from the leaves of S. mole through infusion (referred to as EESM) and its compounds were identified using LC-DAD-MS data. Female rats were then subjected to acute oral toxicity tests using doses of 5, 50, 300, and 2000 mg/kg of ESSM. Studies on genetic material, including the micronucleus test and comet assay, were conducted on male and female Wistar rats using the same doses as in the acute toxicity test. For both assays, ESSM was administered orally.
The main metabolites annotated from ESSM were dimeric proanthocyanidins, phenylpropanoids acids, flavan-3-ols, simple organic acids (C6-C1), a flavonol di-O-glycosylated (rutin), and O-glycosylated megastigmane. The ESSM did not exhibit any acute toxic effects, such as changes in biochemical, hematologic, or histopathological analysis. Furthermore, no changes were observed in comet assay or micronucleus tests when rats were given doses of 5, 50, 300, or 2000 mg/kg of ESSM.
The results showed that the ESSM does not induce acute toxicity or exhibit genotoxicity up to a dose of 2000 mg/kg.
秘鲁乳香是一种漆树科药用植物。它通常被称为“阿罗埃拉”，其叶和根被用于治疗不同的病理状况。然而，尽管它在传统医学中广泛使用，但缺乏深入的毒理学研究。
评估秘鲁乳香水提取物/乙醇可溶部分对大鼠的急性毒性和遗传毒性。
首先，通过浸泡从秘鲁乳香叶中获得纯化的水提取物（称为EESM），并使用LC-DAD-MS数据鉴定其化合物。然后，对雌性大鼠进行急性口服毒性试验，使用剂量为5、50、300和2000mg/kg的EESM。使用与急性毒性试验相同的剂量，对雄性和雌性Wistar大鼠进行包括微核试验和彗星试验在内的遗传物质研究。对于这两种试验，EESM均通过口服给药。
从EESM中鉴定出的主要代谢产物为二聚体原花青素、苯丙烷酸、黄烷-3-醇、简单有机酸（C6-C1）、一种黄酮醇二-O-糖苷（芦丁）和O-糖苷化的巨大戟烷。EESM未表现出任何急性毒性作用，如生化、血液学或组织病理学分析的变化。此外，当给大鼠服用剂量为5、50、300或2000mg/kg的EESM时，在彗星试验或微核试验中未观察到任何变化。
结果表明，在高达2000mg/kg的剂量下，EESM不会诱导急性毒性或表现出遗传毒性。
