Arif Syrine, Larochelle Sébastien, Trudel Benjamin, Gounou Céline, Bordeleau François, Brisson Alain R, Moulin Véronique J
Faculté de Médecine Université Laval Quebec Quebec City Canada.
Centre de Recherche du CHU de Québec-Université Laval Quebec Quebec City Canada.
J Extracell Biol. 2023 Dec 27;3(1):e131. doi: 10.1002/jex2.131. eCollection 2024 Jan.
Microvesicles (MVs) are a subtype of extracellular vesicles that can transfer biological information over long distances, affecting normal and pathological processes including skin wound healing. However, the diffusion of MVs into tissues can be impeded by the extracellular matrix (ECM). We investigated the diffusion of dermal wound myofibroblast-derived MVs into the ECM by using hydrogels composed of different ECM molecules such as fibrin, type III collagen and type I collagen that are present during the healing process. Fluorescent MVs mixed with hydrogels were employed to detect MV diffusion using fluorometric methods. Our results showed that MVs specifically bound type I collagen and diffused freely out of fibrin and type III collagen. Further analysis using flow cytometry and specific inhibitors revealed that MVs bind to type I collagen via the α2β1 integrin. These data demonstrate that MV transport depends on the composition of the wound environment.
微泡（MVs）是细胞外囊泡的一种亚型，能够远距离传递生物信息，影响包括皮肤伤口愈合在内的正常和病理过程。然而，微泡向组织中的扩散会受到细胞外基质（ECM）的阻碍。我们通过使用由愈合过程中存在的不同细胞外基质分子（如纤维蛋白、III型胶原蛋白和I型胶原蛋白）组成的水凝胶，研究了真皮伤口成肌纤维细胞衍生的微泡在细胞外基质中的扩散。将荧光微泡与水凝胶混合，采用荧光法检测微泡扩散。我们的结果表明，微泡特异性结合I型胶原蛋白，并能从纤维蛋白和III型胶原蛋白中自由扩散出来。使用流式细胞术和特异性抑制剂进行的进一步分析表明，微泡通过α2β1整合素与I型胶原蛋白结合。这些数据表明，微泡的运输取决于伤口环境的组成。
