Li Ruimin, Du Yimin, Zhou Yan, Ouyang Zhigang
College of Life Sciences, Gannan Normal University, Ganzhou, 341000, China.
National Navel Orange Engineering Research Center, Ganzhou, 341000, China.
Mol Biotechnol. 2025 Jul;67(7):2709-2719. doi: 10.1007/s12033-024-01232-4. Epub 2024 Jun 28.
Huanglongbing (HLB), a global citrus threat, is transmitted by Diaphorina citri Kuwayama, a widespread insect pest. The disease's rapid spread and incurability necessitate efficient, sustainable control strategies. This study investigates heat shock protein 70 (HSP70) genes in D. citri, known to play a pivotal role in insect survival and stress response. The genome-wide identification, gene structure analysis, and conserved protein domain analysis of 22 HSP70 genes in D. citri were performed. Furthermore, the expression of these genes during HLB infection or developmental processes was gauged. Phylogenetic analysis revealed the functional categorization of the identified genes, while gene structure and conserved motifs offered insights into gene function. The expression analysis unveiled dynamic profiles in response to infection and across development stages, potentially aiding future targeted pest control strategies. These findings offer promising leads for the design of novel inhibitors or RNAi strategies targeting D. citri and HLB.
黄龙病（HLB）是一种全球性的柑橘威胁，由广泛分布的害虫柑橘木虱（Diaphorina citri Kuwayama）传播。该病的迅速传播和无法治愈性使得高效、可持续的控制策略成为必要。本研究调查了柑橘木虱中的热休克蛋白70（HSP70）基因，已知该基因在昆虫生存和应激反应中起关键作用。对柑橘木虱中的22个HSP70基因进行了全基因组鉴定、基因结构分析和保守蛋白结构域分析。此外，还测定了这些基因在HLB感染或发育过程中的表达情况。系统发育分析揭示了所鉴定基因的功能分类，而基因结构和保守基序则为基因功能提供了见解。表达分析揭示了对感染和不同发育阶段的动态反应模式，这可能有助于未来针对性的害虫控制策略。这些发现为设计针对柑橘木虱和HLB的新型抑制剂或RNA干扰策略提供了有希望的线索。
