Edwards J A, Jones D B, Evans P R, Smith J L
Immunology. 1985 Jul;55(3):489-500.
A panel of monoclonal antibodies to monomorphic determinants of the MHC class II subregion locus products: DP, DR and DQ, was used to investigate the expression of these antigens on early lymphocytes and macrophages from human fetal liver (13-20 weeks), placenta (16 weeks and term) and cord blood, in relation to the class II phenotype of cells from adult tonsil and peripheral blood. Fetal liver sections and cell suspensions showed differential expression of class II antigens. DP was expressed at a higher frequency (11.0% of nucleated cells) than DR on lymphoid cells and macrophages from fetal liver, and DQ was either absent or expressed on less than 0.3% of nucleated cells. Consistent with this finding, DP but not DR or DQ antigens were observed on vascular elements and macrophages in the villi of 16-week placenta. At term, all three subregion locus products were expressed. Adult tonsil and peripheral blood B lymphocytes expressed DP, DR and DQ antigens with similar frequency; however, DQ was expressed at a lower frequency than DP and DR on cord blood B lymphocytes. In contrast, 30-50% macrophages from cord blood and adult peripheral blood expressed DP and DR, but fewer (5% and 18%, respectively) expressed DQ. These data suggest that class II antigens are expressed in the sequence DP, DR, DQ on developing lymphocytes. A similar sequence is suggested for macrophages.
一组针对主要组织相容性复合体（MHC）II类亚区基因座产物（DP、DR和DQ）单态性决定簇的单克隆抗体，被用于研究这些抗原在人胎儿肝脏（13 - 20周）、胎盘（16周和足月）及脐血中早期淋巴细胞和巨噬细胞上的表达情况，并与成人扁桃体和外周血中细胞的II类表型进行关联分析。胎儿肝脏切片和细胞悬液显示了II类抗原的差异表达。在胎儿肝脏的淋巴细胞和巨噬细胞上，DP的表达频率（占核细胞的11.0%）高于DR，而DQ要么不表达，要么在少于0.3%的核细胞上表达。与此发现一致，在16周胎盘绒毛的血管成分和巨噬细胞上观察到了DP抗原，但未观察到DR或DQ抗原。足月时，所有三个亚区基因座产物均有表达。成人扁桃体和外周血B淋巴细胞表达DP、DR和DQ抗原的频率相似；然而，脐血B淋巴细胞上DQ的表达频率低于DP和DR。相比之下，脐血和成人外周血中30 - 50%的巨噬细胞表达DP和DR，但表达DQ的较少（分别为5%和18%）。这些数据表明，II类抗原在发育中的淋巴细胞上按DP、DR、DQ的顺序表达。巨噬细胞也呈现出类似的顺序。
