Knapp G, Ogden R C, Peebles C L, Abelson J
Cell. 1979 Sep;18(1):37-45. doi: 10.1016/0092-8674(79)90351-9.
The intermediates of the yeast tRNA splicing reaction have been characterized. The intervening sequence is excised as an unique linear molecule. It has 5'-hydroxyl and 3'-phosphate termini. Correspondingly, the half-tRNA molecules are shown to have a 3'-phosphate terminus on the 5' half and 5'-hydroxyl terminus on the 3' half. These isolated halves have been shown to be active in the ligation step of tRNA splicing. Removal of the 3'-phosphate from the 5' half eliminates the ability of the 5' half to participate in ligation.
酵母tRNA剪接反应的中间体已得到表征。间隔序列作为一个独特的线性分子被切除。它具有5'-羟基和3'-磷酸末端。相应地，半tRNA分子在5'半部分显示有一个3'-磷酸末端，在3'半部分有一个5'-羟基末端。这些分离的半分子已被证明在tRNA剪接的连接步骤中具有活性。从5'半部分去除3'-磷酸会消除5'半部分参与连接的能力。
