State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. China.
A.V. Nikolaev Institute of Inorganic Chemistry, Siberian Branch of the Russian Academy of Sciences, Avenue Lavrentyev 3, Novosibirsk 630090, Russian Federation.
Anal Chem. 2024 Jul 16;96(28):11334-11342. doi: 10.1021/acs.analchem.4c01265. Epub 2024 Jun 29.
Detecting harmful pathogens in food is not only a crucial aspect of food quality management but also an effective way to ensure public health. In this paper, a complete nuclear magnetic resonance biosensor based on a novel gadolinium (Gd)-targeting molecular probe was developed for the detection of Salmonella in milk. First, streptavidin was conjugated to the activated macromolecular polyaspartic acid (PASP) via an amide reaction to generate SA-PASP. Subsequently, the strong chelating and adsorption properties of PASP toward the lanthanide metal gadolinium ions were exploited to generate the magnetic complex (SA-PASP-Gd). Finally, the magnetic complex was linked to biotinylated antibodies to obtain the bioprobe and achieve the capture of Salmonella. Under optimal experimental conditions, the sensor we have constructed can achieve a rapid detection of Salmonella within 1.5 h, with a detection limit of 7.1 × 10 cfu mL.
检测食品中的有害病原体不仅是食品质量管理的关键环节，也是确保公众健康的有效途径。在本文中，我们开发了一种完整的基于新型镧系元素（Gd）靶向分子探针的核磁共振生物传感器，用于检测牛奶中的沙门氏菌。首先，通过酰胺反应将链霉亲和素偶联到活化的高分子聚天冬氨酸（PASP）上，生成 SA-PASP。随后，利用 PASP 对镧系金属钆离子的强螯合和吸附特性生成磁性复合物（SA-PASP-Gd）。最后，将磁性复合物与生物素化抗体连接，得到生物探针，实现对沙门氏菌的捕获。在最佳实验条件下，我们构建的传感器可以在 1.5 小时内快速检测到沙门氏菌，检测限为 7.1×10 cfu·mL。
