Bonfanti C, Meurman O, Halonen P
J Virol Methods. 1985 Jun;11(2):161-70. doi: 10.1016/0166-0934(85)90039-4.
A new solid phase enzyme immunoassay (EIA) for detection of rubella-specific immunoglobulin G (IgG) antibody was developed. The test uses polystyrene microtiter strips coated with rabbit anti-human IgG immunoglobulins as the solid phase and an enzyme-labelled semipurified rubella antigen as indicator. The direct EIA was compared with hemagglutination inhibition (HI), single radial hemolysis (SRH), radioimmunoassay (RIA) and time-resolved fluoroimmunoassay (TR-FIA) using 52 serum specimens from patients with remote rubella infection. The overall agreement of direct EIA with HI was 96.1%, with SRH and RIA 98.1% and with TR-FIA 100%. The linear regression coefficient varied from 0.77 to 0.91, the best being obtained with direct EIA and SRH. The direct EIA was also suitable for diagnosis of acute infections, as a significant increase in antibody levels was detected in all paired specimens tested from patients with acute rubella infection. The sensitivity and were comparable to those of the assays employed. An advantage of the present assay is that the same method and same labelled antigen can be used to test for different classes of antibody using simply a solid phase with capture antibodies of different chain specificity.
研发了一种用于检测风疹特异性免疫球蛋白G（IgG）抗体的新型固相酶免疫测定法（EIA）。该检测使用包被有兔抗人IgG免疫球蛋白的聚苯乙烯微量滴定条作为固相，并用酶标记的半纯化风疹抗原作为指示剂。使用来自远期风疹感染患者的52份血清标本，将直接EIA与血凝抑制试验（HI）、单向辐射溶血试验（SRH）、放射免疫测定法（RIA）和时间分辨荧光免疫测定法（TR-FIA）进行比较。直接EIA与HI的总体一致性为96.1%，与SRH和RIA为98.1%，与TR-FIA为100%。线性回归系数在0.77至0.91之间变化，直接EIA与SRH得到的结果最佳。直接EIA也适用于急性感染的诊断，因为在所有来自急性风疹感染患者的配对标本检测中均检测到抗体水平显著升高。其敏感性与所采用的检测方法相当。本检测方法的一个优点是，使用具有不同链特异性捕获抗体的固相，相同的方法和相同的标记抗原可用于检测不同类别的抗体。
