Zhang Liaoruilin, Xiang Jianguo, Li Juan, Zhou Jie, Hou Jinliang, Huang Yanfei, Li Hong
College of Fisheries, Hunan Agricultural University, No.1 Nongda Road, Furong District, Changsha, Hunan Province,410128, China Hunan Agricultural University Changsha China.
Comp Cytogenet. 2024 Jun 21;18:97-103. doi: 10.3897/compcytogen.18.116806. eCollection 2024.
The current study analyzed the chromosomal karyotype of David, 1875 from Hunan Province, China. The karyotype, C-banding, BrdU-banding pattern were characterized using direct preparation of bone-marrow cells and hemocyte cultures. The findings indicated that was a diploid species (2n = 26) that lacked heteromorphic chromosomes and secondary constrictions. C-banding analysis revealed an abundance of positive signals in the centromere regions, while the BrdU-banding pattern showed three phases in both male and female, occurring consistently and in chronological sequence during S-phase. Notably, there was no asynchronous replication in the late phase. This study enhanced our understanding of the karyotypic structure of by conventional cytogenetic techniques, thus providing essential scientific insights into the cytogenetics of .
本研究分析了采自中国湖南省的大卫氏（1875年）的染色体核型。通过直接制备骨髓细胞和血细胞培养物，对核型、C带、BrdU带型进行了表征。研究结果表明，大卫氏是二倍体物种（2n = 26），缺乏异型染色体和次缢痕。C带分析显示着丝粒区域有大量阳性信号，而BrdU带型在雄性和雌性中均显示三个阶段，在S期按时间顺序一致出现。值得注意的是，后期没有异步复制。本研究通过传统细胞遗传学技术增进了我们对大卫氏核型结构的理解，从而为大卫氏的细胞遗传学提供了重要的科学见解。
