Institute of Optics and Electronics, Chinese Academy of Sciences, P.O. Box 350, Chengdu, 610209, China; School of Optoelectronic Science and Engineering, University of Electronic Science and Technology of China, Chengdu, 610054, China; National Key Laboratory of Optical Field Manipulation Science and Technology, Chinese Academy of Sciences, P.O. Box 350, Chengdu, 610209, China; School of Optoelectronics, University of Chinese Academy of Sciences, Beijing, 100049, China.
Institute of Optics and Electronics, Chinese Academy of Sciences, P.O. Box 350, Chengdu, 610209, China; National Key Laboratory of Optical Field Manipulation Science and Technology, Chinese Academy of Sciences, P.O. Box 350, Chengdu, 610209, China; School of Optoelectronics, University of Chinese Academy of Sciences, Beijing, 100049, China.
Talanta. 2024 Oct 1;278:126494. doi: 10.1016/j.talanta.2024.126494. Epub 2024 Jun 29.
The spread of the SARS-CoV-2 virus has had an unprecedented impact, both by posing a serious risk to human health and by amplifying the burden on the global economy. The rapid identification of the SARS-CoV-2 virus has been crucial to preventing and controlling the spread of SARS-CoV-2 infections. In this study, we propose a multilayered plasmonic nanotrap (MPNT) device for the rapid identification of single particles of SARS-CoV-2 virus in ultra-high sensitivity by surface-enhanced Raman scattering (SERS). The MPNT device is composed of arrays of concentric cylindrical cavities with Ag/SiO/Ag multilayers deposited on the top and at the bottom. By varying the diameter of the cylinders and the thickness of the multilayers, the resonant optical absorption and local electric field were optimized. The SERS enhancement factors of the proposed device are of the order of 10, which enable the rapid identification of SARS-CoV-2 N protein in concentrations as low as 1.25 × 10－12.5 × 10 g mL within 1 min. The developed MPNT SERS device provides a label-free and rapid detection platform for SARS-CoV-2 virus. The general nature of the device makes it equally suitable to detect other infectious viruses.
SARS-CoV-2 病毒的传播产生了前所未有的影响，不仅对人类健康构成严重威胁，还加重了全球经济的负担。快速鉴定 SARS-CoV-2 病毒对于预防和控制 SARS-CoV-2 感染的传播至关重要。在这项研究中，我们提出了一种多层等离子体纳米阱（MPNT）器件，通过表面增强拉曼散射（SERS）实现对 SARS-CoV-2 病毒单颗粒的超高灵敏度快速识别。MPNT 器件由顶部和底部沉积有 Ag/SiO/Ag 多层的同心圆柱形腔阵列组成。通过改变圆柱的直径和多层的厚度，优化了共振光吸收和局域电场。所提出器件的 SERS 增强因子约为 10，可在 1 分钟内快速识别低至 1.25×10-12.5×10g mL浓度的 SARS-CoV-2 N 蛋白。所开发的 MPNT SERS 器件为 SARS-CoV-2 病毒提供了一种无标记和快速的检测平台。该器件的通用性使其同样适用于检测其他传染性病毒。
