The Third Affiliated Hospital of Wenzhou Medical University, Wenzhou 325200, China.
The Third Affiliated Hospital of Wenzhou Medical University, Wenzhou 325200, China.
Int J Biol Macromol. 2024 Aug;275(Pt 1):133557. doi: 10.1016/j.ijbiomac.2024.133557. Epub 2024 Jun 30.
Autologous or allogeneic bone tissue grafts remain the mainstay of treatment for clinical bone defects. However, the risk of infection and donor scarcity in bone grafting pose challenges to the process. Therefore, the development of excellent biomaterial grafts is of great clinical importance for the repair of bone defects. In this study, we used gas-assisted microfluidics to construct double-cross-linked hydrogel microspheres with good biological function based on the ionic cross-linking of Cu with alginate and photo-cross-linking of gelatin methacryloylamide (GelMA) by loading vascular endothelial growth factor (VEGF) and His-tagged bone morphogenetic protein-2 (BMP2) (AGMP@VEGF&BMP2). The Cu component in the microspheres showed good antibacterial and drug-release behavior, whereas VEGF and BMP2 effectively promoted angiogenesis and bone tissue repair. In in vitro and in vivo experiments, the dual cross-linked hydrogel microspheres showed good biological function and biocompatibility. These results demonstrate that AGMP@VEGF&BMP2 microspheres could be used as a bone defect graft substitute to promote effective healing of bone defects and may be applied to other tissue engineering studies.
自体或同种异体骨组织移植物仍然是治疗临床骨缺损的主要方法。然而，骨移植中感染和供体缺乏的风险给该过程带来了挑战。因此，开发优良的生物材料移植物对于修复骨缺损具有重要的临床意义。在本研究中，我们使用气体辅助微流控技术，基于藻酸盐与铜的离子交联和明胶甲基丙烯酰胺（GelMA）的光交联，构建了具有良好生物学功能的双交联水凝胶微球，负载血管内皮生长因子（VEGF）和 His 标记的骨形态发生蛋白-2（BMP2）（AGMP@VEGF&BMP2）。微球中的 Cu 成分表现出良好的抗菌和药物释放行为，而 VEGF 和 BMP2 则有效促进了血管生成和骨组织修复。在体外和体内实验中，双交联水凝胶微球表现出良好的生物学功能和生物相容性。这些结果表明，AGMP@VEGF&BMP2 微球可用作骨缺损移植物替代物，以促进骨缺损的有效愈合，并可能应用于其他组织工程研究。
