Jahan Esrat, Mazumder Tanoy, Hasan Tarek, Ahmed Khondoker Shahin, Amanat Muhammed, Hossain Hemayet, Supty Sumaiya Jannat, Liya Israt Jahan, Shuvo Md Sadikur Rahman, Daula A F M Shahid Ud
Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali, Bangladesh.
Chemical Research Division, Bangladesh Council of Scientific and Industrial Research, Dhaka, Bangladesh.
Biochem Genet. 2024 Jul 2. doi: 10.1007/s10528-024-10869-4.
The advent of the new coronavirus, leading to the SARS-CoV-2 pandemic, has presented a substantial worldwide health hazard since its inception in the latter part of 2019. The severity of the current pandemic is exacerbated by the occurrence of re-infection or co-infection with SARS-CoV-2. Hence, comprehending the molecular process underlying the pathophysiology of sepsis and discerning possible molecular targets for therapeutic intervention holds significant importance. For the first time, 31 metabolites were tentatively identified by GC-MS analysis from Alpinia malaccensis. On the other hand, five phenolic compounds were identified and quantified from the plant in HPLC-DAD analysis, including (-) epicatechin, rutin hydrate, rosmarinic acid, quercetin, and kaempferol. Nine GC-MS and five HPLC-identified metabolites had shown interactions with 45 and 30 COVID-19-associated human proteins, respectively. Among the proteins, PARP1, FN1, PRKCA, EGFR, ALDH2, AKR1C3, AHR, and IKBKB have been found as potential therapeutic targets to mitigate SARS-CoV-2 infection. KEGG pathway analysis also showed a strong association of FN1, EGFR, and IKBKB genes with SARS-CoV-2 viral replication and cytokine overexpression due to viral infection. Protein-protein interaction (PPI) analysis also showed that TP53, MMP9, FN1, EGFR, and NOS2 proteins are highly related to the genes involved in COVID-19 comorbidity. These proteins showed interaction with the plant phytoconstituents as well. As the study offers a robust network-based procedure for identifying biomolecules relevant to COVID-19 disease, A. malaccensis could be a good source of effective therapeutic agents against COVID-19 and related viral diseases.
自2019年末新型冠状病毒出现并引发SARS-CoV-2大流行以来，它已在全球范围内构成重大健康威胁。SARS-CoV-2再感染或合并感染的发生加剧了当前大流行的严重程度。因此，了解脓毒症病理生理学的分子过程并确定可能的治疗干预分子靶点具有重要意义。通过气相色谱-质谱联用（GC-MS）分析，首次从马六甲良姜中初步鉴定出31种代谢物。另一方面，通过高效液相色谱-二极管阵列检测（HPLC-DAD）分析从该植物中鉴定并定量了5种酚类化合物，包括（-）表儿茶素、水合芦丁、迷迭香酸、槲皮素和山奈酚。GC-MS鉴定出的9种代谢物和HPLC鉴定出的5种代谢物分别与45种和30种与COVID-19相关的人类蛋白质发生了相互作用。在这些蛋白质中，聚（ADP-核糖）聚合酶1（PARP1）、纤连蛋白1（FN1）、蛋白激酶Cα（PRKCA）、表皮生长因子受体（EGFR）、乙醛脱氢酶2（ALDH2）、醛酮还原酶1C3（AKR1C3）、芳烃受体（AHR）和核因子κB抑制蛋白激酶β（IKBKB）已被发现是减轻SARS-CoV-2感染的潜在治疗靶点。京都基因与基因组百科全书（KEGG）通路分析还显示，FN1、EGFR和IKBKB基因与SARS-CoV-2病毒复制以及病毒感染导致的细胞因子过表达密切相关。蛋白质-蛋白质相互作用（PPI）分析还表明，TP53、基质金属蛋白酶9（MMP9）、FN1、EGFR和一氧化氮合酶2（NOS2）蛋白与COVID-19合并症相关基因高度相关。这些蛋白质也与植物的植物成分发生了相互作用。由于该研究提供了一种基于网络的强大程序来识别与COVID-19疾病相关的生物分子，马六甲良姜可能是对抗COVID-19及相关病毒疾病的有效治疗剂的良好来源。
