This paper presents a comprehensive review of recent advancements in utilizing zinc oxide nanoparticles (ZnO NPs) to enhance antimicrobial and UV protective properties in healthcare solutions. It delves into the synthesis techniques of ZnO NPs and elucidates their antimicrobial efficacy, exploring the underlying mechanisms governing their action against a spectrum of pathogens. Factors impacting the antimicrobial performance of ZnO NPs, including size, surface characteristics, and environmental variables, are extensively analyzed. Moreover, recent studies showcasing the effectiveness of ZnO NPs against diverse pathogens are critically examined, underscoring their potential utility in combatting microbial infections. The study further investigates the UV protective capabilities of ZnO NPs, elucidating the mechanisms by which they offer UV protection and reviewing recent innovations in leveraging them for UV-blocking applications in healthcare. It also dissects the factors influencing the UV shielding performance of ZnO NPs, such as particle size, dispersion quality, and surface coatings. Additionally, the paper addresses challenges associated with integrating ZnO NPs into healthcare products and presents future perspectives for overcoming these hurdles. It emphasizes the imperative for continued research efforts and collaborative initiatives to fully harness the potential of ZnO NPs in developing advanced healthcare solutions with augmented antimicrobial and UV protective attributes. By advancing our understanding and leveraging innovative approaches, ZnO NPs hold promise for addressing pressing healthcare needs and enhancing patient care outcomes.