Mochamad Lazuardi, Malarvili Selvaraja, Jasmine Khairat, Lim Vuanghao
Sub-division Veterinary Pharmacy Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, 60115, Indonesia.
Faculty of Pharmaceutical Sciences, UCSI University, No.1, Jalan Menara Gading, Taman Connaught, Kuala Lumpur, Wilayah Persekutuan, Malaysia.
F1000Res. 2024 Jul 23;12:1214. doi: 10.12688/f1000research.133489.2. eCollection 2023.
Recent evidence suggests that some flavonoid compounds obtained from crude methanol extract of mistletoe leaves ( ), also known as Benalu Duku (BD), have antimicrobial effects. Thus, the plant has the potential to eliminate viruses that may cause outbreaks in chicken farms. This study aimed to prove the ability of flavonoid compounds, namely quercetin-like compounds (QLCs), to eliminate field viruses, specifically the Newcastle disease virus (NDV).
This research was performed in two stages. An test was used with a post-test of the control groups designed at a significance of 0.05. BD leaves (5 kg) were extracted using a maceration method with methanol and then separated into hexane, chloroform, ethyl acetate, and methanol fractions. The final extracted products were separated using semi-preparative high-performance liquid chromatography (HPLC) to obtain QLCs. The QLCs were identified and compared with quercetin using HPLC, proton and carbon nuclear magnetic resonance spectrometry, Fourier transform infrared spectrophotometry and ultra-performance liquid chromatography-mass spectrometry. The activity of QLCs was tested against the NDV at a virulence titter of 10 Tissue Culture Infectious Dose 50% (TCID50) in chicken kidney cell culture.
Solutions of 0.05% (w/v) QLCs were discovered to have antiviral activity against NDVs, with an average cytopathogenic effect antigenicity at a 10 dilution (p<0.05).
QLCs from flavonoids from the leaves of BD have antiviral bioactivity against NDV at a virulence titter of 10-5 Tissue Culture Infectious Dose 50% (TCID50) in chicken kidney cell culture. QLCs may have the potential to be developed as medicinal compounds for the treatment of other human or animal viral infections.
最近的证据表明，从槲寄生叶粗甲醇提取物中获得的一些黄酮类化合物（也称为贝纳鲁·杜库（BD））具有抗菌作用。因此，这种植物有可能消除可能在养鸡场引发疫情的病毒。本研究旨在证明黄酮类化合物，即槲皮素样化合物（QLCs）消除野外病毒，特别是新城疫病毒（NDV）的能力。
本研究分两个阶段进行。采用一项试验，并对对照组进行后测，显著性水平设定为0.05。用甲醇通过浸渍法提取5千克BD叶，然后分离成己烷、氯仿、乙酸乙酯和甲醇馏分。最终提取物通过半制备高效液相色谱（HPLC）分离以获得QLCs。使用HPLC、质子和碳核磁共振光谱、傅里叶变换红外光谱和超高效液相色谱 - 质谱对QLCs进行鉴定并与槲皮素进行比较。在鸡肾细胞培养中，以10个组织培养感染剂量50%（TCID50）的毒力滴度测试QLCs对NDV的活性。
发现0.05%（w/v）的QLCs溶液对NDV具有抗病毒活性，在10倍稀释时平均细胞病变效应抗原性（p<0.05）。
BD叶黄酮类化合物中的QLCs在鸡肾细胞培养中对毒力滴度为10 - 5组织培养感染剂量50%（TCID50）的NDV具有抗病毒生物活性。QLCs可能有潜力被开发为治疗其他人类或动物病毒感染的药用化合物。
