Key Laboratory of Analytical Science for Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350108, PR China.
National Engineering Research Center for Carbohydrate Synthesis, Key Lab of Fluorine and Silicon for Energy Materials and Chemistry of Ministry of Education, Key Laboratory for Green Chemistry of Jiangxi Province, Department of Chemistry and chemical engineering, Jiangxi Normal University, Nanchang 330022, PR China.
Anal Chem. 2024 Jul 16;96(28):11463-11471. doi: 10.1021/acs.analchem.4c01912. Epub 2024 Jul 4.
In this work, we reported a cholesterol oxidase (Chox)-loaded platinum (Pt) nanozyme with the collaborative cascade nanoreactor for the construction of nanozyme-enzyme-linked immunosorbent assay (N-ELSA) models to realize high-throughput rapid evaluation of cancer markers. Considering the high specific surface area and manipulable surface sites, ZIF-8 was used as a substrate for natural enzyme and nanozyme loading. The constructed ZIF-8-Pt nanozyme platform exhibited efficient enzyme-like catalytic efficiency with a standard corrected activity of 60.59 U mg, which was 12 times higher than that of the ZIF-8 precursor, and highly efficient photothermal conversion efficiency (∼35.49%). In N-ELISA testing, developed multienzyme photothermal probes were immobilized in microplates based on antigen-antibody-specific reactions. Cholesterol was reacted in a cascade to reactive oxygen radicals, which attacked 3,3',5,5'-tetramethylbenzidine, causing it to oxidize and color change, thus exhibiting highly enhanced efficient photothermal properties. Systematic temperature evaluations were performed by a hand-held microelectromechanical system thermal imager under the excitation of an 808 nm surface light source to determine the cancer antigen 15-3 (CA15-3) profiles in the samples. Encouragingly, the temperature signal from the microwells increased with increasing CA15-3, with a linear range of 2 mU mL to 100 U mL, considering it to be the sensor with the widest working range for visualization and portability available. This work provides new horizons for the development of efficient multienzyme portable colorimetric-photothermal platforms to help advance the community-based process of early cancer detection.
在这项工作中，我们报道了一种负载胆固醇氧化酶（Chox）的铂（Pt）纳米酶，该纳米酶与协同级联纳米反应器一起构建纳米酶-酶联免疫吸附测定（N-ELISA）模型，以实现高通量快速评估癌症标志物。考虑到高比表面积和可操控的表面位点，ZIF-8 被用作天然酶和纳米酶负载的底物。构建的 ZIF-8-Pt 纳米酶平台表现出高效的类酶催化效率，标准校正活性为 60.59 U mg，是 ZIF-8 前体的 12 倍，并且具有高效的光热转换效率（~35.49%）。在 N-ELISA 测试中，开发的多酶光热探针基于抗原-抗体特异性反应被固定在微孔板中。胆固醇在级联反应中与活性氧反应，活性氧攻击 3,3',5,5'-四甲基联苯胺，使其氧化并发生颜色变化，从而表现出高度增强的高效光热特性。通过手持式微机电系统热像仪在 808nm 表面光源的激发下进行系统温度评估，以确定样品中的癌抗原 15-3（CA15-3）谱。令人鼓舞的是，随着 CA15-3 的增加，微井中的温度信号增加，线性范围为 2 mU mL 至 100 U mL，考虑到它是具有最宽工作范围的可视化和便携式传感器。这项工作为开发高效多酶便携式比色-光热平台提供了新的思路，有助于推进基于社区的早期癌症检测过程。
