Honghui Tang, Yifei Wang, Sihan Bian, Yanqing Mao, Lin Wang
Center of Clinical Laboratory and Translational Medicine, The Fourth Affiliated Hospital of Soochow University, Suzhou Dushu Lake Hospital, Suzhou, 215028, China.
Health Management Center, The Fourth Affiliated Hospital of Soochow University, Suzhou Dushu Lake Hospital, Suzhou, 215028, China.
Pract Lab Med. 2025 Jul 6;46:e00490. doi: 10.1016/j.plabm.2025.e00490. eCollection 2025 Sep.
To explore pre-analysis factors affecting the results of neuron-specific enolase (NSE) testing of serum samples, select optimal testing conditions, and reduce the false-positive rate of NSE.
Serum NSE levels were measured using the Roche e801 electrochemiluminescence immunoassay in healthy participants who underwent physical examinations at the Fourth Affiliated Hospital of Soochow University from September 2022 to December 2024, including both outpatients and hospitalized patients. Further, we analyzed the effects of various pre-analytical factors on the NSE positivity rate (NSE>16.3 ng/mL was judged to be NSE-positive) and the proportion of hemolysis index (H) ≥13 (H = 13 was equivalent to hemoglobin concentration of 13 mg/dl).
This study found that the positive rate of NSE and the H ≥ 13 proportion were influenced by factors such as hemolysis, the method of sample transportation, pre-centrifugation storage time, the brand of blood collection tube, and centrifugation conditions. However, post-centrifugation storage time had little effect on these results.
After blood collection, the NSE positive rate and the H ≥ 13 proportion significantly decreased using a gentler transportation method, such as a box logistics or manual transport, centrifuging within 30-40 min or no longer than 1 h at the latest post-collection, and centrifuging at 2100 (acceleration 4, deceleration 6) and 20 °C for 10 min with brand B blood collection tubes. Under these conditions, there was no significant impact on NSE results for males with H < 6 and females with H < 5.
探讨影响血清样本神经元特异性烯醇化酶（NSE）检测结果的分析前因素，选择最佳检测条件，降低NSE的假阳性率。
采用罗氏e801电化学发光免疫分析法检测2022年9月至2024年12月在苏州大学第四附属医院进行体检的健康参与者（包括门诊患者和住院患者）的血清NSE水平。此外，我们分析了各种分析前因素对NSE阳性率（NSE>16.3 ng/mL判定为NSE阳性）和溶血指数（H）≥13（H = 13相当于血红蛋白浓度13 mg/dl）比例的影响。
本研究发现，NSE阳性率和H≥13比例受溶血、样本运输方式、离心前储存时间、采血管品牌和离心条件等因素影响。然而，离心后储存时间对这些结果影响较小。
采血后，采用较温和的运输方式，如盒装物流或人工运输，在采血后30 - 40分钟内或最晚不超过1小时离心，并使用B品牌采血管在2100（加速度4，减速度6）和20°C下离心10分钟，NSE阳性率和H≥13比例显著降低。在这些条件下，对于H < 6的男性和H < 5的女性，对NSE结果无显著影响。
