Nantong University Medical School, Nantong 226019, Jiangsu, China.
Nantong Key Laboratory of Public Health and Medical Analysis, School of Public Health, Nantong University, Nantong 226019, Jiangsu, China.
Anal Chem. 2024 Sep 3;96(35):14116-14124. doi: 10.1021/acs.analchem.4c01570. Epub 2024 Jul 5.
The H1N1 influenza virus is a significant pathogen responsible for seasonal influenza, and its frequent outbreaks pose substantial challenges to global public health. The present study successfully developed a lateral flow analysis platform that integrates reverse transcription-free exponential amplification reaction (RTF-EXPAR) and hybridization chain reaction (HCR) processes with functionalized quantum dots for the direct detection of H1N1 influenza virus RNA, eliminating the need for reverse transcription. The fluorescence signal on the band recorded with a smartphone can be utilized for the quantitative determination of the target. Interestingly, the dual signal amplification strategy exhibits high sensitivity with a remarkably low detection limit of 10 aM. Moreover, this platform exhibits excellent flexibility and universality, where the various pathogens can be determined by replacing the specific nucleic acid fragments in RTF-EXPAR. The aforementioned advantages reveal its huge potential in the early diagnosis of H1N1 influenza virus infection and developing point-of-care testing (POCT) equipment for nucleic acid analysis.
甲型 H1N1 流感病毒是一种重要的病原体，可导致季节性流感，其频繁爆发给全球公共卫生带来了重大挑战。本研究成功开发了一种侧向流动分析平台，该平台集成了无逆转录指数扩增反应（RTF-EXPAR）和杂交链式反应（HCR）过程，并结合功能化量子点，可直接检测 H1N1 流感病毒 RNA，无需逆转录。用智能手机记录的条带上的荧光信号可用于定量测定目标。有趣的是，双信号扩增策略具有很高的灵敏度，检测限低至 10 aM。此外，该平台具有出色的灵活性和通用性，通过替换 RTF-EXPAR 中的特定核酸片段，可以确定各种病原体。上述优点表明其在甲型 H1N1 流感病毒感染的早期诊断和开发用于核酸分析的即时检测（POCT）设备方面具有巨大的潜力。
