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本文引用的文献

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Rapid-test sensitivity for novel swine-origin influenza A (H1N1) virus in humans.新型猪源甲型流感（H1N1）病毒在人类中的快速检测敏感性。
N Engl J Med. 2009 Aug 13;361(7):728-9. doi: 10.1056/NEJMc0904264. Epub 2009 Jun 29.
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Detection of novel (swine origin) H1N1 influenza A virus by quantitative real-time reverse transcription-PCR.通过定量实时逆转录聚合酶链反应检测新型（猪源）甲型H1N1流感病毒
J Clin Microbiol. 2009 Aug;47(8):2675-7. doi: 10.1128/JCM.01087-09. Epub 2009 Jun 24.
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Development of a real-time RT-PCR for the detection of swine-lineage influenza A (H1N1) virus infections.用于检测猪源甲型流感（H1N1）病毒感染的实时逆转录聚合酶链反应技术的开发。
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Analytical sensitivity of rapid influenza antigen detection tests for swine-origin influenza virus (H1N1).针对猪源甲型流感病毒（H1N1）的快速流感抗原检测试验的分析灵敏度
J Clin Virol. 2009 Jul;45(3):205-7. doi: 10.1016/j.jcv.2009.05.034. Epub 2009 Jun 6.
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Origins and evolutionary genomics of the 2009 swine-origin H1N1 influenza A epidemic.2009年甲型H1N1猪源流感疫情的起源与进化基因组学
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Detection of novel influenza A(H1N1) virus by real-time RT-PCR.通过实时逆转录聚合酶链反应检测新型甲型H1N1流感病毒
J Clin Virol. 2009 Jul;45(3):203-4. doi: 10.1016/j.jcv.2009.05.032. Epub 2009 Jun 6.
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Antigenic and genetic characteristics of swine-origin 2009 A(H1N1) influenza viruses circulating in humans.在人群中传播的源自猪的2009年甲型H1N1流感病毒的抗原和基因特征
Science. 2009 Jul 10;325(5937):197-201. doi: 10.1126/science.1176225. Epub 2009 May 22.
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Molecular detection of a novel human influenza (H1N1) of pandemic potential by conventional and real-time quantitative RT-PCR assays.通过常规和实时定量逆转录聚合酶链反应检测具有大流行潜力的新型人类甲型流感（H1N1）病毒
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Triple-reassortant swine influenza A (H1) in humans in the United States, 2005-2009.2005 - 2009年美国人类感染的三重重配甲型H1流感病毒
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Emergence of a novel swine-origin influenza A (H1N1) virus in humans.一种新型猪源甲型流感病毒（H1N1）在人类中的出现。
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建立逆转录环介导等温扩增检测技术用于检测大流行（H1N1）2009 病毒作为一种新的分子方法用于资源有限的地方的大流行性流感的诊断。

Development of a reverse transcription-loop-mediated isothermal amplification assay for detection of pandemic (H1N1) 2009 virus as a novel molecular method for diagnosis of pandemic influenza in resource-limited settings.

机构信息

Center for International Collaborative Research, Institute for Tropical Medicine, Nagasaki University, 1-12-4, Sakamoto, Nagasaki 852-8523, Japan.

出版信息

J Clin Microbiol. 2010 Mar;48(3):728-35. doi: 10.1128/JCM.01481-09. Epub 2010 Jan 13.

DOI:10.1128/JCM.01481-09
PMID:20071551
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2832456/
Abstract

This paper reports on the development of a one-step, real-time reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay targeting the hemagglutinin (HA) gene for the rapid molecular-based detection of pandemic (H1N1) 2009 virus. The detection limit of the pandemic (H1N1) 2009 virus HA-specific RT-LAMP assay was same as that of the currently used real-time reverse transcription-PCR method. The assay detected the pandemic (H1N1) 2009 virus HA gene in 136 RNA samples extracted from nasopharyngeal swab specimens from Japanese and Vietnamese patients. No cross-reactive amplification with the RNA of other seasonal influenza viruses was observed, and the detection of specific viral genome targets in clinical specimens was achieved in less than 40 min. The sensitivity and specificity of the pandemic (H1N1) 2009 virus HA-specific RT-LAMP assay obtained in this study were 97.8% and 100%, respectively. Use of the (H1N1) 2009 virus HA-specific RT-LAMP assay will enable the faster and easier diagnosis of pandemic (H1N1) 2009 virus infection, especially in resource-limited situations in developing countries.

摘要

本文报道了一种针对血凝素 (HA) 基因的一步法、实时逆转录环介导等温扩增 (RT-LAMP) 检测方法的开发，用于快速基于分子的检测大流行 (H1N1) 2009 病毒。大流行 (H1N1) 2009 病毒 HA 特异性 RT-LAMP 检测方法的检测限与目前使用的实时逆转录-PCR 方法相同。该检测方法在从日本和越南患者鼻咽拭子样本中提取的 136 个 RNA 样本中检测到大流行 (H1N1) 2009 病毒 HA 基因。未观察到与其他季节性流感病毒的 RNA 发生交叉反应性扩增，在不到 40 分钟的时间内即可在临床标本中检测到特定病毒基因组靶标。本研究中获得的大流行 (H1N1) 2009 病毒 HA 特异性 RT-LAMP 检测方法的灵敏度和特异性分别为 97.8%和 100%。使用 (H1N1) 2009 病毒 HA 特异性 RT-LAMP 检测方法将能够更快、更容易地诊断大流行 (H1N1) 2009 病毒感染，特别是在发展中国家资源有限的情况下。