Department of Chemistry, Faculty of Science, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand; Futuristic Science Research Center, School of Science, Walailak University, Thasala, Nakhon Si Thammarat 80160, Thailand.
Department of Chemistry, Faculty of Science, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Dec 5;322:124706. doi: 10.1016/j.saa.2024.124706. Epub 2024 Jun 23.
Two novel fluorescent probes for Cu detection have been developed based on thiazoline-quinoline conjugates bearing a 4-ethynyl-N,N-dimethylaniline unit (QT1 and QT2). QT2 exhibits instantaneous fluorescence quenching of Cu with an emissive change from bright orange to arctic blue under UV light irradiation (365 nm). The plots of I/I against Cu concentrations show a good linear relationship that ranges from 0 to 50 µM with a coefficient of determination (R) = 0.9906 and a limit of detection (LOD) of 76 nM, which is considered low (4.84 ppb). A 1:1 complexation between QT2 and Cu was confirmed by UV-Vis titration, ESI-MS, and SC-XRD. The QT2·Cu complex was dissociated by the addition of EDTA. The fluorescence quenching mechanism involves the ligand-to-metal charge transfer (LMCT) of a paramagnetic Cu complex. The QT2 probe on a paper-based strip was used to determine the amount of Cu in water and food samples (shiitake mushrooms and oysters).
基于噻唑啉-喹啉共轭物，设计了两种用于 Cu 检测的新型荧光探针，其带有 4-乙炔基-N,N-二甲基苯胺单元（QT1 和 QT2）。QT2 在紫外光（365nm）照射下，Cu 瞬时荧光猝灭，发光颜色由亮橙色变为冰蓝色。I/I 与 Cu 浓度的关系图显示出良好的线性关系，范围从 0 到 50µM，决定系数（R）= 0.9906，检测限（LOD）为 76nM，这被认为是低的（4.84ppb）。通过紫外-可见滴定、ESI-MS 和 SC-XRD 证实了 QT2 与 Cu 之间形成了 1:1 的络合物。加入 EDTA 后，QT2·Cu 络合物发生解离。荧光猝灭机制涉及顺磁性 Cu 配合物的配体到金属电荷转移（LMCT）。基于纸基条带的 QT2 探针可用于测定水样和食物样（香菇和牡蛎）中的 Cu 含量。
