Farcas Radu A, Almasri Malaz, Grad Simona, Popa Stefan-Lucian, Leucuta Daniel C, Ismaiel Abdulrahman, Dumitrascu Dan L
2nd Department of Internal Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Dr Constantin Papilian Cluj Napoca Emergency Military Hospital, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj Napoca, Romania.
J Neurogastroenterol Motil. 2024 Jul 30;30(3):281-289. doi: 10.5056/jnm24013.
BACKGROUND/AIMS: Disruptions in tight junction (TJ) protein expression leading to duodenal epithelial barrier impairment may contribute to increased intestinal permeability, potentially playing a role in functional dyspepsia (FD) pathophysiology. Currently published studies evaluated the role of several TJ proteins in FD patients with inconsistent results. Therefore, we conducted this systematic review and metaanalysis to evaluate the duodenal mucosal expression of several TJ proteins in FD.
We performed a systematic electronic search on PubMed, EMBASE, and Scopus using predefined keywords. Diagnosis of FD by Rome III or Rome IV criteria was considered acceptable. Full articles satisfying our inclusion and exclusion criteria were included. The principal summary outcome was the mean difference of several TJ proteins in FD patients and control subjects.
A total of 8 and 5 studies were included in our qualitative and quantitative synthesis, respectively, with a total population of 666 participants, out of which 420 were FD patients. No significant differences were observed between FD patients and controls in the expression of claudin-1 (-0.102 [95% CI, -0.303, 0.099]), claudin-2 (0.161 [95% CI, -0.134, 0.456)], claudin-3 (0.278 [95% CI, -0.280, 0.837]), claudin-4 (0.045 [95% CI, -0.264, 0.354]), ZO-1 (-0.221 [95% CI, -0.683, 0.241]), ZO-2 (-0.070 [95% CI, -0.147, 0.007]), ZO-3 (-0.129 [95% CI, -0.376, 0.118]), β-catenin (-0.135 [95% CI, -0.484, 0.214]), E-cadherin (-0.083 [95% CI, -0.229, 0.063]), and occludin (-0.158 [95% CI, -0.409, 0.093]).
The expressions of all evaluated proteins including claudin-1, claudin-2, claudin-3, claudin-4, ZO-1, ZO-2, ZO-3, β-catenin, E-cadherin, and occludin did not significantly differ between FD patients and controls. However, due to the limited number of included studies, results should be interpreted with caution.
背景/目的：紧密连接（TJ）蛋白表达的破坏导致十二指肠上皮屏障受损，可能会增加肠道通透性，这在功能性消化不良（FD）的病理生理学中可能起一定作用。目前已发表的研究评估了几种TJ蛋白在FD患者中的作用，但结果并不一致。因此，我们进行了这项系统评价和荟萃分析，以评估FD患者十二指肠黏膜中几种TJ蛋白的表达情况。
我们使用预定义的关键词在PubMed、EMBASE和Scopus上进行了系统的电子检索。采用罗马III或罗马IV标准诊断FD被认为是可接受的。纳入符合我们纳入和排除标准的全文。主要汇总结果是FD患者和对照受试者中几种TJ蛋白的平均差异。
我们的定性和定量综合分析分别纳入了8项和5项研究，总共有666名参与者，其中420名是FD患者。在FD患者和对照组之间，紧密连接蛋白-1（-0.102 [95%置信区间，-0.303，0.099]）、紧密连接蛋白-2（0.161 [95%置信区间，-0.134，0.456]）、紧密连接蛋白-3（0.278 [95%置信区间，-0.280，0.837]）、紧密连接蛋白-4（0.045 [95%置信区间，-0.264，0.354]）、闭合蛋白-1（-0.221 [95%置信区间，-0.683，0.241]）、闭合蛋白-2（-0.070 [95%置信区间，-0.147，0.007]）、闭合蛋白-3（-0.129 [95%置信区间，-0.376，0.118]）、β-连环蛋白（-0.135 [95%置信区间，-0.484，0.214]）、E-钙黏蛋白（-0.083 [95%置信区间，-0.229，0.063]）和闭合蛋白（-0.158 [95%置信区间，-0.409，0.093]）的表达没有显著差异。
包括紧密连接蛋白-1、紧密连接蛋白-2、紧密连接蛋白-3、紧密连接蛋白-4、闭合蛋白-1、闭合蛋白-2、闭合蛋白-3、β-连环蛋白、E-钙黏蛋白和闭合蛋白在内的所有评估蛋白的表达在FD患者和对照组之间没有显著差异。然而，由于纳入研究的数量有限，结果应谨慎解释。
