Department of Biological Science, College of Science and Engineering, Sangji University, Wonju, 26339, Republic of Korea.
Department of Environmental Engineering, College of Science and Technology, Korea University Sejong Campus, 2511 Sejong-ro, Sejong City, 30019, Republic of Korea.
Int J Syst Evol Microbiol. 2023 Nov;73(11). doi: 10.1099/ijsem.0.006171.
A milky-white-coloured, aerobic, Gram-stain-positive, rod-shaped and motile bacterial strain (GW78) was isolated from forest soil. GW78 was catalase-positive and oxidase-negative. The strain was able to grow optimally at 37 °C and at pH 7.0 in Reasoner's 2A media. The phylogenetic and 16S rRNA gene sequence analysis of GW78 showed its affiliation with the genus . The 16S rRNA gene sequence of GW78 revealed 98.3 % similarity to its nearest neighbour VKPM B-7519. Its chemotaxonomic properties included MK-7 as the sole menaquinone, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylmonomethylethanolamine and phosphatidylethanolamine as major polar lipids, and anteiso-C, C 11 and anteiso-C as predominant fatty acids. Digital DNA-DNA hybridization and average nucleotide identity results with its closest relatives were <74.0 % and <14.0 %, respectively. Overall, 16S rRNA gene sequence comparisons, phylogenetic and genomic evidence, and phenotypic and chemotaxonomic data allow the differentiation of GW78 from other members of the genus . Thus, we propose that strain GW78 represents a novel species of the genus , with the name sp. nov. The type strain is GW78 (=KCTC 43430=NBRC 116023).
一株乳白色、需氧、革兰氏阳性、杆状且运动的细菌（GW78）从森林土壤中分离得到。GW78 为过氧化氢酶阳性和氧化酶阴性。该菌株在 Reasoner 2A 培养基中最适生长温度为 37°C，pH 值为 7.0。GW78 的系统发育和 16S rRNA 基因序列分析表明，它属于 属。GW78 的 16S rRNA 基因序列与最接近的近缘种 VKPM B-7519 具有 98.3%的相似性。其化学分类特征包括 MK-7 作为唯一的menaquinone，磷脂酰甘油、双磷脂酰甘油、磷脂酰单甲基乙醇胺和磷脂酰乙醇胺作为主要极性脂，以及 anteiso-C11 和 anteiso-C15 作为主要脂肪酸。与最接近的亲缘关系的数字 DNA-DNA 杂交和平均核苷酸同一性结果分别为<74.0%和<14.0%。总的来说，16S rRNA 基因序列比较、系统发育和基因组证据以及表型和化学分类数据允许 GW78 与属的其他成员区分开来。因此，我们建议菌株 GW78 代表一个新的 属物种，命名为 sp. nov.。模式菌株为 GW78（=KCTC 43430=NBRC 116023）。
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