Woo Yu Kyong, Kang Minseong, Kim Changhee, Hwang Jae-Kwan
Graduate Program in Bioindustrial Engineering, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea.
Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea.
Prev Nutr Food Sci. 2024 Jun 30;29(2):154-161. doi: 10.3746/pnf.2024.29.2.154.
Skeletal muscle atrophy, which is characterized by diminished muscle mass, strength, and function, is caused by malnutrition, physical inactivity, aging, and diseases. Korean mint ( Kuntze) possesses various biological functions, including anti-inflammatory, antioxidant, anticancer, and antiosteoporosis activities. Moreover, it contains tilianin, which is a glycosylated flavone that exerts antioxidant, anti-inflammatory, antidiabetic, and neuroprotective activities. However, no studies have analyzed the inhibitory activity of extract (ARE) and tilianin on muscle atrophy. Thus, the present study investigated the potential of ARE and tilianin on muscle atrophy and their underlying mechanisms of action in C2C12 myotubes treated with tumor necrosis factor-α (TNF-α). The results showed that ARE and tilianin promoted the phosphatidylinositol 3-kinase/protein kinase B pathway, thereby activating mammalian target of rapamycin (a protein anabolism-related factor) and its downstream factors. Moreover, ARE and tilianin inhibited the mRNA expression of muscle RING-finger protein-1 and atrogin-1 (protein catabolism-related factors) by blocking Forkhead box class O translocation. ARE and tilianin also mitigated inflammatory responses by downregulating nuclear factor-kappa B expression levels, thereby diminishing the expression levels of inflammatory cytokines, including TNF-α and interleukin-6. Additionally, ARE and tilianin enhanced the expression levels of antioxidant enzymes, including catalase, superoxide dismutase, and glutathione peroxidase. Overall, these results suggest that ARE and tilianin are potential functional ingredients for preventing or improving muscle atrophy.
骨骼肌萎缩的特征是肌肉质量、力量和功能下降，其由营养不良、缺乏身体活动、衰老和疾病引起。薄荷（唇萼薄荷）具有多种生物学功能，包括抗炎、抗氧化、抗癌和抗骨质疏松活性。此外，它含有田蓟苷，这是一种糖基化黄酮，具有抗氧化、抗炎、抗糖尿病和神经保护活性。然而，尚无研究分析薄荷提取物（ARE）和田蓟苷对肌肉萎缩的抑制活性。因此，本研究调查了ARE和田蓟苷对肌肉萎缩的潜在作用及其在肿瘤坏死因子-α（TNF-α）处理的C2C12肌管中的潜在作用机制。结果表明，ARE和田蓟苷促进磷脂酰肌醇3-激酶/蛋白激酶B通路，从而激活雷帕霉素靶蛋白（一种与蛋白质合成代谢相关的因子）及其下游因子。此外，ARE和田蓟苷通过阻断叉头框O类易位来抑制肌肉环指蛋白-1和atrogin-1（与蛋白质分解代谢相关的因子）的mRNA表达。ARE和田蓟苷还通过下调核因子-κB表达水平来减轻炎症反应，从而降低包括TNF-α和白细胞介素-6在内的炎症细胞因子的表达水平。此外，ARE和田蓟苷提高了包括过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶在内的抗氧化酶的表达水平。总体而言，这些结果表明，ARE和田蓟苷是预防或改善肌肉萎缩的潜在功能性成分。
