Bone-Muscle Research Center, College of Nursing and Health Innovations, Department of Graduate Nursing, The University of Texas at Arlington, Arlington, TX, USA.
Spinal Cord Damage Research Center, James J. Peters VA Medical Center, Bronx, NY, USA.
Methods Mol Biol. 2024;2816:87-100. doi: 10.1007/978-1-0716-3902-3_9.
Laparotomy (EL) is one of the most common procedures performed among surgical specialties. Previous research demonstrates that surgery is associated with an increased inflammatory response. Low psoas muscle mass and quality markers are associated with increased mortality rates after emergency laparotomy. Analysis of lipid mediators in serum and muscle by using liquid chromatography-mass spectrometry (LC-MS)-based lipidomics has proven to be a sensitive and precise technique. In this chapter, we describe an LC-MS/MS protocol for the profiling and quantification of signaling lipids formed from Eicosapentaenoic Acid (EPA) and Eicosatetranoic acid (ETA) by 5, 12, or 15 lipoxynases. This protocol has been developed for and validated in serum and muscle samples in a mouse model of surgical stress caused by laparotomy.
剖腹术（EL）是外科专业中最常见的手术之一。先前的研究表明，手术与炎症反应增加有关。在急诊剖腹术后，较低的腰大肌质量和质量标志物与较高的死亡率相关。使用基于液相色谱-质谱（LC-MS）的脂质组学分析血清和肌肉中的脂质介质已被证明是一种敏感且精确的技术。在本章中，我们描述了一种 LC-MS/MS 方案，用于分析和定量由 5、12 或 15 型脂氧合酶从二十碳五烯酸（EPA）和二十碳四烯酸（ETA）形成的信号脂质。该方案已针对由剖腹术引起的手术应激的小鼠模型中的血清和肌肉样本进行了开发和验证。
