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[快速自动细菌鉴定及药敏分析系统。4小时内完成抗生素检测的新系统。描述及变异参数]

[Rapid ATB. A new system of antibiotic testing in 4 hours. Description and parameters of variation].

作者信息

Gayral J P, Carret G, Flandrois J P, Le Noc P, Robert J, Albertini M T, Gallice E

出版信息

Pathol Biol (Paris). 1985 May;33(5):350-3.

PMID:3897964
Abstract

Rapid ATB is a new automated system which performs antibiotic sensitivity testing of Enterobacteriaceae in four hours. It is composed of strips containing dehydrated antibiotics and a medium which ensures rapid bacterial growth, and of the equipment required for standardization of the inoculum, reading of results and interpretation. Strains are classified as susceptible or resistant according to the value of the growth index (IC) which is the ratio of growth in the presence of the antibiotic to growth of a control inoculum. Use of this system requires standardization. The main factors to control are state of the culture used to prepare the inoculum, standardization of the inoculum, incubation temperature (35 to 37 degrees C), and duration of incubation (4 to 5 hours). Variations of these factors usually induce significantly delayed growth. Under well standardized conditions, results are highly reproducible, as proved by testing ten strains ten times each by three investigators.

摘要

快速自动细菌鉴定及药敏分析系统(Rapid ATB)是一种新型自动化系统,可在4小时内完成肠杆菌科细菌的抗生素敏感性检测。它由含有脱水抗生素的试条和确保细菌快速生长的培养基,以及用于接种物标准化、结果读取和判读所需的设备组成。菌株根据生长指数(IC)的值分为敏感或耐药,生长指数是抗生素存在下的生长与对照接种物生长的比值。使用该系统需要标准化。需要控制的主要因素包括用于制备接种物的培养物状态、接种物的标准化、孵育温度(35至37摄氏度)和孵育持续时间(4至5小时)。这些因素的变化通常会导致生长显著延迟。在标准化良好的条件下,结果具有高度可重复性,三位研究人员对10株菌株各进行10次测试证明了这一点。

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Pathol Biol (Paris). 1985 May;33(5):350-3.
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