Gayral J P, Albertini M T, Gallice E, Olléon M
Pathol Biol (Paris). 1986 May;34(5):364-7.
Rapid ATB Staph is a method for four-hour antimicrobial susceptibility testing of staphylococci. The equipment and theoretical basis are identical to those already described for rapid testing of enterobacteriaceae. A standardized procedure is required to perform the test. The inoculum should be prepared from a culture no older than 24 hours and accurately standardized at 10(8). Temperature of incubation should be 35 degrees C to 37 degrees C; otherwise growth is delayed by approximately one hour. Time of incubation should not exceed 5 hours. Using these standardized conditions, rapid ATB Staph was compared with the reference agar dilution method for 22 antibiotics. Overall agreement was 96.3%. The rapid system is more sensitive than the reference method fort the detection of netilmicin and amikacin resistance; it is reliable for detecting penicillin resistance, oxacillin resistance and inducible resistance to erythromycin (agreement 96.7%, 94.8% and 98.4% respectively). A Rapid ATB Staph strip containing 15 antibiotics has been designed for routine susceptibility testing.
快速葡萄球菌鉴定药敏试验（Rapid ATB Staph）是一种对葡萄球菌进行4小时抗菌药敏试验的方法。其设备和理论基础与已描述的用于肠杆菌科细菌快速检测的相同。进行该试验需要标准化程序。接种物应取自培养不超过24小时的培养物，并准确标准化至10⁸。孵育温度应为35℃至37℃；否则生长会延迟约1小时。孵育时间不应超过5小时。在这些标准化条件下，将快速葡萄球菌鉴定药敏试验与参考琼脂稀释法对22种抗生素进行了比较。总体符合率为96.3%。该快速系统在检测奈替米星和阿米卡星耐药性方面比参考方法更敏感；在检测青霉素耐药性、苯唑西林耐药性和诱导性红霉素耐药性方面是可靠的（符合率分别为96.7%、94.8%和98.4%）。已设计出一种包含15种抗生素的快速葡萄球菌鉴定药敏试验条带用于常规药敏试验。
