Plisetskaya E, Pollock H G, Rouse J B, Hamilton J W, Kimmel J R, Gorbman A
Regul Pept. 1985 Jun;11(2):105-16. doi: 10.1016/0167-0115(85)90071-0.
Insulin has been isolated from islet tissue of coho salmon (Oncorhynchus kisutch) by gel filtration and HPLC and the complete amino acid sequence has been determined. The sequence differs from bovine insulin at 14 sites but all interchanges are conservative from the viewpoint of preservation of conformation. A comparison of insulin sequences from other fish is presented. Salmon insulin cross-reacts very weakly with antiserum to bovine insulin and vice versa. A completely homologous radioimmunoassay has been developed and used to estimate the insulin in salmon islet tissue and in plasma. The hypoglycemic effect of salmon insulin in salmon was more pronounced and persisted longer than that caused by identical doses of bovine insulin.
已通过凝胶过滤和高效液相色谱法从银大麻哈鱼（Oncorhynchus kisutch）的胰岛组织中分离出胰岛素，并确定了其完整的氨基酸序列。该序列与牛胰岛素在14个位点不同，但从构象保留的角度来看，所有替换都是保守的。文中还对其他鱼类的胰岛素序列进行了比较。鲑鱼胰岛素与抗牛胰岛素血清的交叉反应非常弱，反之亦然。已开发出一种完全同源的放射免疫分析法，并用于估算鲑鱼胰岛组织和血浆中的胰岛素。鲑鱼胰岛素在鲑鱼体内的降血糖作用比相同剂量的牛胰岛素更明显，且持续时间更长。
