银大麻哈鱼（Oncorhynchus kisutch）胰高血糖素和胰高血糖素样肽的分离与结构

Isolation and structures of coho salmon (Oncorhynchus kisutch) glucagon and glucagon-like peptide.

作者信息

Plisetskaya E, Pollock H G, Rouse J B, Hamilton J W, Kimmel J R, Gorbman A

出版信息

Regul Pept. 1986 Mar;14(1):57-67. doi: 10.1016/0167-0115(86)90205-3.

PMID:3520699

Abstract

Glucagon and glucagon-like peptide (GLP) containing 31 amino acids have been isolated from the principal islet of coho salmon (Oncorhynchus kisutch) by gel filtration of acid alcohol extracts followed by HPLC, and the complete amino acid sequence of both peptides has been determined. Salmon glucagon is a simple 29 residue peptide differing at 3 positions when compared to catfish glucagon and at 8 positions when compared to porcine glucagon. Salmon GLP differs at 6 positions when compared with the N-terminal 31 amino acids of the 34 amino acid catfish GLP. Both coho salmon glucagon and GLP cross-react weakly in our mammalian glucagon radioimmunoassay and therefore this technique could not be used to determine tissue content. Glucagon and GLP isolated amounted to 156 micrograms/g and 350 micrograms/g wet tissue, respectively.

摘要

通过对酸醇提取物进行凝胶过滤，随后进行高效液相色谱（HPLC），从银大麻哈鱼（Oncorhynchus kisutch）的主要胰岛中分离出了含有31个氨基酸的胰高血糖素和胰高血糖素样肽（GLP），并确定了这两种肽的完整氨基酸序列。与鲶鱼胰高血糖素相比，鲑鱼胰高血糖素是一种简单的由29个残基组成的肽，在3个位置上存在差异；与猪胰高血糖素相比，在8个位置上存在差异。与34个氨基酸的鲶鱼GLP的N端31个氨基酸相比，鲑鱼GLP在6个位置上存在差异。在我们的哺乳动物胰高血糖素放射免疫测定中，银大麻哈鱼的胰高血糖素和GLP的交叉反应都很弱，因此该技术无法用于测定组织含量。分离出的胰高血糖素和GLP分别为每克湿组织156微克和350微克。