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外泌体miRNA-21-5p和miRNA-21-3p作为心肌梗死的关键生物标志物。

Exosomal miRNA-21-5p and miRNA-21-3p as key biomarkers of myocardial infarction.

作者信息

Sahebi Reza, Gandomi Fatemeh, Shojaei Mitra, Farrokhi Effat

机构信息

Department of Molecular Medicine, School of Advanced Technologies Shahrekord University of Medical Sciences Shahrekord Iran.

Metabolic Syndrome Research Center, School of Medicine Mashhad University of Medical Sciences Mashhad Iran.

出版信息

Health Sci Rep. 2024 Jul 9;7(7):e2228. doi: 10.1002/hsr2.2228. eCollection 2024 Jul.

DOI:10.1002/hsr2.2228
PMID:38983683
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11232052/
Abstract

OBJECTIVE

Coronary artery disease (CAD) is a debilitating condition that can lead to myocardial infarction (MI). Exosomal miRNAs (exo-miRNA) can be diagnostic biomarkers for detecting MI. Here, we conduct a study to evaluate the efficacy of exo-miRNA-21-5p/3p for early detection of MI.

METHODS

A total of 135 CAD patients and 150 healthy subjects participated in this study. Additionally, we randomly divided 26 male Wistar rats (12 weeks old) into two groups: control and induced MI. Angiographic images were used to identify patients and healthy individuals of all genders. In the following, serum exosomes were obtained, and exo-miRNA-21-5p/3p was measured by reverse-transcriptase polymerase chain reaction.

RESULTS

We observed an upregulation of exo-miRNA-21-5p/3p in CAD patient and MI-induced animal groups compared to controls. Analysis of the ROC curves defined 82% and 88% of the participants' exo-miRNA-21-5p and exo-miRNA-21-3p diagnostic power, respectively, which in the animal model was 92 and 82.

CONCLUSION

This study revealed that the mean expression levels of exo-miRNA-21-5p/3p were significantly increased in CAD patients and animal models of induced MI. Also, these results are associated with the atherogenic lipid profile of CAD patients, which may play an important role in the progression of the disease. Therefore, they can be considered as novel biomarkers.

摘要

目的

冠状动脉疾病（CAD）是一种使人衰弱的病症，可导致心肌梗死（MI）。外泌体微小RNA（外泌体-miRNA）可作为检测MI的诊断生物标志物。在此，我们开展一项研究以评估外泌体-miRNA-21-5p/3p用于MI早期检测的效果。

方法

共有135例CAD患者和150名健康受试者参与了本研究。此外，我们将26只12周龄的雄性Wistar大鼠随机分为两组：对照组和诱导MI组。利用血管造影图像识别所有性别的患者和健康个体。接下来，获取血清外泌体，并通过逆转录聚合酶链反应测定外泌体-miRNA-21-5p/3p。

结果

与对照组相比，我们观察到CAD患者组和MI诱导动物组中外泌体-miRNA-21-5p/3p上调。ROC曲线分析确定参与者的外泌体-miRNA-21-5p和外泌体-miRNA-21-3p的诊断能力分别为82%和88%，在动物模型中分别为92%和82%。

结论

本研究显示，CAD患者和MI诱导动物模型中外泌体-miRNA-21-5p/3p的平均表达水平显著升高。此外，这些结果与CAD患者的致动脉粥样硬化血脂谱相关，这可能在疾病进展中起重要作用。因此，它们可被视为新型生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec5/11232052/e632ba01916a/HSR2-7-e2228-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec5/11232052/e3e4a6ec5dfb/HSR2-7-e2228-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec5/11232052/52a63f15e499/HSR2-7-e2228-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec5/11232052/a6c9c25ecb3e/HSR2-7-e2228-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec5/11232052/d723f0032e7c/HSR2-7-e2228-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec5/11232052/2e546b0c831f/HSR2-7-e2228-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec5/11232052/728c85caff8b/HSR2-7-e2228-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec5/11232052/e632ba01916a/HSR2-7-e2228-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec5/11232052/e3e4a6ec5dfb/HSR2-7-e2228-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec5/11232052/52a63f15e499/HSR2-7-e2228-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec5/11232052/a6c9c25ecb3e/HSR2-7-e2228-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec5/11232052/d723f0032e7c/HSR2-7-e2228-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec5/11232052/2e546b0c831f/HSR2-7-e2228-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec5/11232052/728c85caff8b/HSR2-7-e2228-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ec5/11232052/e632ba01916a/HSR2-7-e2228-g003.jpg

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