Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany.
Acta Crystallogr D Struct Biol. 2024 Aug 1;80(Pt 8):563-579. doi: 10.1107/S2059798324005588. Epub 2024 Jul 10.
Serial crystallography, born from groundbreaking experiments at the Linac Coherent Light Source in 2009, has evolved into a pivotal technique in structural biology. Initially pioneered at X-ray free-electron laser facilities, it has now expanded to synchrotron-radiation facilities globally, with dedicated experimental stations enhancing its accessibility. This review gives an overview of current developments in serial crystallography, emphasizing recent results in time-resolved crystallography, and discussing challenges and shortcomings.
连续结晶学起源于 2009 年在林可相干光源（Linac Coherent Light Source）的开创性实验，现已发展成为结构生物学的关键技术。该技术最初由 X 射线自由电子激光设施开创，现已扩展到全球同步辐射设施，并设有专用实验站以提高其可及性。本综述概述了连续结晶学的最新发展，强调了在时间分辨结晶学方面的最新成果，并讨论了挑战和缺点。
