Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, México.
Unidad de Biología Integrativa, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, México.
Methods Mol Biol. 2024;2827:145-153. doi: 10.1007/978-1-0716-3954-2_9.
Plant cell suspension cultures (PCSCs) are in vitro-cultured cells that can divide indefinitely in a sterile growth medium. These PCSCs can be derived from various plant tissues, such as the root, stem, leaves, or seeds, and are maintained in a suitable culture medium containing nutrients, vitamins, hormones, and other essential components necessary for their growth. PCSCs have extensive applications in biotechnology, particularly in producing pharmaceutical and chemical compounds. This chapter presents a protocol for generating cell lines from Arabidopsis thaliana root callus under different light conditions, which can be used to investigate the effects of light on plant cell growth and development. The protocol described in this chapter is a valuable tool for researchers interested in utilizing PCSCs in their studies.
植物细胞悬浮培养物（PCSCs）是在无菌生长培养基中可以无限分裂的体外培养细胞。这些 PCSCs 可以从各种植物组织中获得，例如根、茎、叶或种子，并在含有营养物、维生素、激素和其他必要成分的合适培养基中维持。PCSCs 在生物技术中有广泛的应用，特别是在生产药物和化学化合物方面。本章介绍了在不同光照条件下从拟南芥根愈伤组织中生成细胞系的方案，可用于研究光照对植物细胞生长和发育的影响。本章中描述的方案是对利用 PCSCs 进行研究的研究人员有价值的工具。
