Max-Planck-Institute of Molecular Plant Physiology, Potsdam-Golm, Germany.
Methods Mol Biol. 2024;2827:405-416. doi: 10.1007/978-1-0716-3954-2_27.
The engineering of plant cell cultures to produce high-value natural products is suggested to be a safe, low-cost, and environmentally friendly route to produce a wide range of chemicals. Given that the expression of heterologous biosynthetic pathways in plant tissue culture is limited by a lack of detailed protocols, the biosynthesis of high-value metabolites in plant cell culture is constrained compared with that in microbes. However, both Arabidopsis thaliana and Nicotiana benthamiana can be efficiently transformed with multigene constructs to produce high-value natural products in stable plant cell cultures. This chapter provides a detailed protocol as to how to engineer the plant cell culture as bio-factories for metabolite biosynthesis.
将植物细胞培养工程化以生产高价值天然产物被认为是一种安全、低成本和环保的方法，可用于生产广泛的化学品。鉴于异源生物合成途径在植物组织培养中的表达受到缺乏详细方案的限制，与微生物相比，植物细胞培养中高价值代谢物的生物合成受到限制。然而，拟南芥和本氏烟都可以通过多基因构建体高效转化，在稳定的植物细胞培养物中生产高价值的天然产物。本章提供了一个详细的方案，说明如何将植物细胞培养工程化为生物合成代谢物的生物工厂。
