Earlham Institute, Norwich Research Park, Norwich, NR4 7UZ, UK.
Earlham Institute, Norwich Research Park, Norwich, NR4 7UZ, UK; Department of Plant Sciences, University of Cambridge, Cambridge, CB2 3EA, UK.
Curr Opin Plant Biol. 2024 Oct;81:102611. doi: 10.1016/j.pbi.2024.102611. Epub 2024 Aug 3.
Nicotiana benthamiana is a model plant, widely used for research. The susceptibility of young plants to Agrobacterium tumefaciens has been utilised for transient gene expression, enabling the production of recombinant proteins at laboratory and commercial scales. More recently, this technique has been used for the rapid prototyping of synthetic genetic circuits and for the elucidation and reconstruction of metabolic pathways. In the last few years, many complex metabolic pathways have been successfully reconstructed in this species. In addition, the availability of improved genomic resources and efficient gene editing tools have enabled the application of sophisticated metabolic engineering approaches to increase the purity and yield of target compounds. In this review, we discuss recent advances in the use of N. benthamiana for understanding and engineering plant metabolism, as well as efforts to improve the utility of this species as a production chassis for natural products.
黄花烟是一种模式植物，广泛用于研究。幼嫩植物对根癌农杆菌的易感性已被用于瞬时基因表达，使重组蛋白在实验室和商业规模上得以生产。最近，该技术已被用于合成遗传回路的快速原型设计，以及代谢途径的阐明和重建。在过去的几年中，许多复杂的代谢途径已在该物种中成功重建。此外，改进的基因组资源和高效的基因编辑工具的可用性，使复杂的代谢工程方法得以应用，以提高目标化合物的纯度和产量。在这篇综述中，我们讨论了利用黄花烟理解和工程植物代谢的最新进展，以及努力提高该物种作为天然产物生产底盘的实用性。
