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Green technologies for the extraction of proteins from jackfruit leaves ( Lam).从菠萝蜜叶(Lam)中提取蛋白质的绿色技术。
Food Sci Biotechnol. 2020 Oct 6;29(12):1675-1684. doi: 10.1007/s10068-020-00825-4. eCollection 2020 Dec.
2
Characterization and antifungal activity of jackfruit (Artocarpus heterophyllus Lam.) leaf extract obtained using conventional and emerging technologies.采用常规和新兴技术对菠萝蜜(Artocarpus heterophyllus Lam.)叶提取物进行的特性分析及抗真菌活性研究。
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Ultrasonic microwave-assisted extraction coupled with macroporous resin chromatography for the purification of antioxidant phenolics from waste jackfruit ( Lam.) peels.超声微波辅助萃取结合大孔树脂色谱法从废弃菠萝蜜(Lam.)果皮中纯化抗氧化酚类物质。
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Edible pectin film added with peptides from jackfruit leaves obtained by high-hydrostatic pressure and pepsin hydrolysis.添加了通过高静水压和胃蛋白酶水解获得的波罗蜜叶肽的可食用果胶膜。
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本文引用的文献

1
Carotenoid extraction methods: A review of recent developments.类胡萝卜素提取方法:近期进展综述
Food Chem. 2018 Feb 1;240:90-103. doi: 10.1016/j.foodchem.2017.07.099. Epub 2017 Jul 20.
2
Quantification of major allergen parvalbumin in 22 species of fish by SDS-PAGE.用 SDS-PAGE 定量分析 22 种鱼类中的主要过敏原 parvalbumin。
Food Chem. 2016 Mar 1;194:345-53. doi: 10.1016/j.foodchem.2015.08.037. Epub 2015 Aug 12.
3
Salting-out and salting-in: competitive effects of salt on the aggregation behavior of soy protein particles and their emulsifying properties.盐析和盐溶:盐对大豆蛋白颗粒聚集行为及其乳化特性的竞争效应
Soft Matter. 2015 Aug 7;11(29):5926-32. doi: 10.1039/c5sm00954e.
4
Effects of high hydrostatic pressure (HHP) on the protein structure and thermal stability of Sauvignon blanc wine.超高压处理对长相思白葡萄酒蛋白质结构和热稳定性的影响。
Food Chem. 2014 Jul 15;155:214-20. doi: 10.1016/j.foodchem.2014.01.051. Epub 2014 Jan 25.
5
A study on the enthalpy-entropy compensation in protein unfolding.蛋白质解折叠过程中的焓-熵补偿研究。
Biophys Chem. 2000 May 15;84(3):239-51. doi: 10.1016/s0301-4622(00)00130-7.
6
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.一种利用蛋白质 - 染料结合原理对微克级蛋白质进行定量的快速灵敏方法。
Anal Biochem. 1976 May 7;72:248-54. doi: 10.1016/0003-2697(76)90527-3.

从菠萝蜜叶(Lam)中提取蛋白质的绿色技术。

Green technologies for the extraction of proteins from jackfruit leaves ( Lam).

作者信息

Moreno-Nájera Laura Cristina, Ragazzo-Sánchez Juan Arturo, Gastón-Peña Cristina Regla, Calderón-Santoyo Montserrat

机构信息

National Technologic of Mexico/Technologic Institute of Tepic, 2595 Tecnologico Av., 63175 Tepic, Nayarit Mexico.

AZCUBA Sugar Group, 171 23th Av., Havana, Cuba.

出版信息

Food Sci Biotechnol. 2020 Oct 6;29(12):1675-1684. doi: 10.1007/s10068-020-00825-4. eCollection 2020 Dec.

DOI:10.1007/s10068-020-00825-4
PMID:33282434
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7708546/
Abstract

The application of emerging technologies such as ultrasound, microwaves and high hydrostatic pressure, allows the extraction of compounds in a sustainable manner from a vegetable matrix with a high value such as jackfruit leaf proteins ( Lam). Currently, the main method of protein extraction is based on the precipitation with the use of an aggressive solvent, therefore it is necessary to optimize extraction methods with a minimum waste production. In the protein extraction of jackfruit leaves, we obtained a content of 84.1 mg/g using solvents. On the other hand, emergent extractions such as ultrasound, microwaves, and high hydrostatic pressure showed concentrations of 96.3, 95.6 and 147.3 mg/g, respectively. In addition, we found that the best extraction agent was 0.5 M NaCl, offering a range of possibilities that support green technologies as an imperative change in the food industry.

摘要

诸如超声波、微波和高静水压等新兴技术的应用,使得从诸如菠萝蜜叶蛋白(Lam)这样具有高价值的植物基质中以可持续的方式提取化合物成为可能。目前,蛋白质提取的主要方法是基于使用腐蚀性溶剂进行沉淀,因此有必要优化提取方法以减少废物产生。在菠萝蜜叶的蛋白质提取中,使用溶剂时我们获得的含量为84.1毫克/克。另一方面,诸如超声波、微波和高静水压等新兴提取方法分别显示出的浓度为96.3毫克/克、95.6毫克/克和147.3毫克/克。此外,我们发现最佳提取剂是0.5M氯化钠,这提供了一系列可能性,支持绿色技术成为食品工业中势在必行的变革。