Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Centre of Oral Biomaterial & Stomatological Key laboratory of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350002, China; Institute of Stomatology & Laboratory of Oral Tissue Engineering, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350002, China.
Shengli Clinical Medical College of Fujian Medical University, Department of Emergency, Fujian Provincial Hospital, Fuzhou 350001, China; Fujian Provincial Key Laboratory of Emergency Medicine, Fujian Provincial Institute of Emergency Medicine, Fujian Emergency Medical Centre, Fuzhou 350001, China.
Int J Biol Macromol. 2024 Sep;276(Pt 1):133775. doi: 10.1016/j.ijbiomac.2024.133775. Epub 2024 Jul 8.
Barrier membranes play a prominent role in guided bone regeneration (GBR), and polycaprolactone (PCL) is an attractive biomaterial for the fabrication of barrier membranes. However, these nanofiber membranes (NFMs) require modification to improve their biological activity. PCL-NFMs incorporating with laponite (LAP) achieve biofunctional modification. Decellularized extracellular matrix (dECM) could modulate cell behaviour. The present study combined dECM with PCL/LAP-NFMs to generate a promising strategy for bone tissue regeneration. Bone marrow mesenchymal stem cells (BMSCs) were cultured on NFMs and deposited with an abundant extracellular matrix (ECM), which was subsequently decellularized to obtain dECM-modified PCL/LAP-NFMs (PCL/LAP-dECM-NFMs). The biological functions of the membranes were evaluated by reseeding MC3T3-E1 cells in vitro and transplanting them into rat calvarial defects in vivo. These results indicate that PCL/LAP-dECM-NFMs were successfully constructed. The presence of dECM slightly improved the mechanical properties of the NFMs, which exhibited a Young's modulus of 0.269 MPa, ultimate tensile strength of 2.04 MPa and elongation at break of 51.62 %. In vitro, the PCL/LAP-dECM-NFMs had favourable cytocompatibility, and the enhanced hydrophilicity was conducive to cell adhesion, proliferation, and osteoblast differentiation. PCL/LAP-dECM-NFMs exhibited an excellent bone repair capacity in vivo. Overall, dECM-modified PCL/LAP-NFMs should be promising biomimetic barrier membranes for GBR.
屏障膜在引导骨再生（GBR）中起着重要作用，聚己内酯（PCL）是制造屏障膜的有吸引力的生物材料。然而，这些纳米纤维膜（NFMs）需要改性以提高其生物活性。掺入拉蓬土（LAP）的 PCL-NFMs 实现了生物功能改性。去细胞细胞外基质（dECM）可以调节细胞行为。本研究将 dECM 与 PCL/LAP-NFMs 结合，为骨组织再生提供了一种有前途的策略。骨髓间充质干细胞（BMSCs）在 NFMs 上培养并沉积丰富的细胞外基质（ECM），随后进行去细胞化以获得 dECM 改性的 PCL/LAP-NFMs（PCL/LAP-dECM-NFMs）。通过体外再接种 MC3T3-E1 细胞并将其移植到大鼠颅骨缺损中，评估了膜的生物学功能。结果表明成功构建了 PCL/LAP-dECM-NFMs。dECM 的存在略微改善了 NFMs 的机械性能，其杨氏模量为 0.269 MPa，拉伸强度为 2.04 MPa，断裂伸长率为 51.62%。体外，PCL/LAP-dECM-NFMs 具有良好的细胞相容性，增强的亲水性有利于细胞黏附、增殖和成骨细胞分化。PCL/LAP-dECM-NFMs 在体内表现出优异的骨修复能力。总体而言，dECM 改性的 PCL/LAP-NFMs 应为 GBR 有前途的仿生屏障膜。
