Mechanobiology and Regenerative Medicine Laboratory, Bioengineering College, Chongqing University, 174 Shazheng Street, Shapingba District, Chongqing 400044, P. R. China.
J Mater Chem B. 2021 Sep 14;9(34):6881-6894. doi: 10.1039/d1tb00895a. Epub 2021 Aug 16.
Extracellular matrix (ECM)-based materials have been employed as scaffolds for bone tissue engineering, providing a suitable microenvironment with biophysical and biochemical cues for cell attachment, proliferation and differentiation. In this study, bone-derived ECM (bECM)-incorporated electrospun poly(ε-caprolactone) (PCL) (bECM/PCL) nanofibrous scaffolds were prepared and their effects on osteogenesis were evaluated in vitro and in vivo. The results showed that the bECM/PCL scaffolds promoted the attachment, spreading, proliferation and osteogenic differentiation of rat mesenchymal stem cells (MSCs), mitigated the foreign-body reaction, and facilitated bone regeneration in a rat calvarial critical size defect model. Thus, this study suggests that bECM can provide a promising option for bone regeneration.
细胞外基质（ECM）基材料已被用作骨组织工程的支架，为细胞附着、增殖和分化提供了合适的具有生物物理和生物化学线索的微环境。在这项研究中，制备了骨衍生细胞外基质（bECM）掺入的静电纺聚己内酯（PCL）（bECM/PCL）纳米纤维支架，并在体外和体内评估了它们的成骨作用。结果表明，bECM/PCL 支架促进了大鼠间充质干细胞（MSCs）的附着、铺展、增殖和成骨分化，减轻了异物反应，并促进了大鼠颅骨临界大小缺损模型中的骨再生。因此，本研究表明 bECM 可为骨再生提供一种有前途的选择。
