Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key laboratory of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, 350002, China.
Institute of Stomatology & Laboratory of Oral Tissue Engineering, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, 350002, China.
Macromol Biosci. 2022 Feb;22(2):e2100265. doi: 10.1002/mabi.202100265. Epub 2021 Nov 8.
Massive oral and maxillofacial bone defect regeneration remains a major clinical challenge due to the absence of functionalized bone grafts with ideal mechanical and proregeneration properties. In the present study, Laponite (LAP), a synthetic nanosilicate, is incorporated into polycaprolactone (PCL) to develop a biomaterial for bone regeneration. It is explored whether LAP-embedded PCL would accelerate bone regeneration by orchestrating osteoblasts to directly and indirectly induce bone regeneration processes. The results confirmed the presence of LAP in PCL, and LAP is distributed in the exfoliated structure without aggregates. Incorporation of LAP in PCL slightly improved the compressive properties. LAP-embedded PCL is biocompatible and exerts pronounced enhancements in cell viability, osteogenic differentiation, and extracellular matrix formation of osteoblasts. Furthermore, osteoblasts cultured on LAP-embedded PCL facilitate angiogenesis of vessel endothelial cells and alleviate osteoclastogenesis of osteoclasts in a paracrine manner. The addition of LAP to the PCL endows favorable bone formation in vivo. Based upon these results, LAP-embedded PCL shows great potential as an ideal bone graft that exerts both space-maintaining and vascularized bone regeneration synergistic effects and can be envisioned for oral and maxillofacial bone defect regeneration.
由于缺乏具有理想机械性能和促进再生性能的功能化骨移植物，大面积口腔颌面部骨缺损的再生仍然是一个主要的临床挑战。在本研究中，将合成纳米硅酸盐 Laponite（LAP）掺入聚己内酯（PCL）中，开发用于骨再生的生物材料。研究是否通过协调成骨细胞直接和间接地诱导骨再生过程，使 LAP 嵌入 PCL 能够加速骨再生。结果证实了 LAP 存在于 PCL 中，并且 LAP 分布在没有聚集物的剥落结构中。LAP 的掺入略微改善了 PCL 的压缩性能。LAP 嵌入 PCL 具有生物相容性，并显著增强了成骨细胞的细胞活力、成骨分化和细胞外基质形成。此外，在 LAP 嵌入 PCL 上培养的成骨细胞通过旁分泌方式促进血管内皮细胞的血管生成并减轻破骨细胞的破骨细胞生成。LAP 添加到 PCL 中赋予了体内良好的骨形成。基于这些结果，LAP 嵌入 PCL 显示出巨大的潜力，作为一种理想的骨移植物，具有维持空间和血管化骨再生的协同作用，可用于口腔颌面部骨缺损的再生。
