Department of Microbiology, Faculty of Biological Sciences, Islamic Azad University, North Tehran Branch, Tehran, Iran.
Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
BMC Complement Med Ther. 2024 Jul 10;24(1):258. doi: 10.1186/s12906-024-04576-1.
Inflammatory bowel disease (IBD) is a chronic, relapsing inflammatory disorder of the gastrointestinal system. So far, no treatment has been identified that can completely cure IBD. Lactobacillus brevis is hypothesized to be beneficial in preventing inflammation. This study aimed to evaluate the potential probiotic effects of live and pasteurized L. brevis IBRC-M10790 on the in vitro cell co-culture model of IBD.
An in vitro intestinal model was established using a transwell co-culture system of Caco-2 intestinal epithelial cells and RAW264.7 macrophages. Inflammatory conditions were induced in RAW264.7 cells using lipopolysaccharide. The effects of live and pasteurized L. brevis IBRC-M10790 on inflammatory mediators and epithelial barrier markers were investigated.
L. brevis IBRC-M10790 was able to significantly decrease the proinflammatory cytokines (IL-6, IL-1β, and TNF-α) and increase the anti-inflammatory cytokine (IL-10) in the in vitro co-culture system. In addition, L. brevis increased adherens and tight junction (TJ) markers (ZO-1, E-cadherin, and Occludin) in Caco-2 intestinal epithelial cells. Based on the results, pasteurized L. brevis showed a higher protective effect than live L. brevis.
Our findings suggest that live and pasteurized forms of L. brevis possess probiotic properties and can mitigate inflammatory conditions in IBD.
炎症性肠病（IBD）是一种慢性、复发性胃肠道炎症性疾病。到目前为止，还没有发现可以完全治愈 IBD 的方法。短双歧杆菌被认为对预防炎症有益。本研究旨在评估活的和巴氏杀菌的短双歧杆菌 IBRC-M10790 对 IBD 体外细胞共培养模型的潜在益生菌作用。
使用 Caco-2 肠上皮细胞和 RAW264.7 巨噬细胞的 Transwell 共培养系统建立体外肠道模型。使用脂多糖诱导 RAW264.7 细胞发生炎症。研究了活的和巴氏杀菌的短双歧杆菌 IBRC-M10790 对炎症介质和上皮屏障标志物的影响。
短双歧杆菌 IBRC-M10790 能够显著降低体外共培养系统中的促炎细胞因子（IL-6、IL-1β 和 TNF-α），并增加抗炎细胞因子（IL-10）。此外，短双歧杆菌增加了 Caco-2 肠上皮细胞中的黏附连接和紧密连接（TJ）标志物（ZO-1、E-钙黏蛋白和Occludin）。基于这些结果，巴氏杀菌的短双歧杆菌比活的短双歧杆菌表现出更高的保护作用。
我们的研究结果表明，活的和巴氏杀菌的短双歧杆菌具有益生菌特性，可以减轻 IBD 中的炎症状态。
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