College of Chemistry and Chemical Engineering, College of Energy Material and Chemistry, Inner Mongolia Key Laboratory of Fine Organic Synthesis, Inner Mongolia University, Hohhot, 010021, China.
Department of Urology, The First Affiliated Hospital of Shandong First Medical University, Jinan, 250000, Shandong, China.
Nat Commun. 2024 Jul 11;15(1):5832. doi: 10.1038/s41467-024-50202-6.
While second near-infrared (NIR-II) fluorescence imaging is a promising tool for real-time surveillance of surgical operations, the previously reported organic NIR-II luminescent materials for in vivo imaging are predominantly activated by expensive lasers or X-ray with high power and poor illumination homogeneity, which significantly limits their clinical applications. Here we report a white-light activatable NIR-II organic imaging agent by taking advantages of the strong intramolecular/intermolecular D-A interactions of conjugated Y6CT molecules in nanoparticles (Y6CT-NPs), with the brightness of as high as 13315.1, which is over two times that of the brightest laser-activated NIR-II organic contrast agents reported thus far. Upon white-light activation, Y6CT-NPs can achieve not only in vivo imaging of hepatic ischemia reperfusion, but also real-time monitoring of kidney transplantation surgery. During the surgery, identification of the renal vasculature, post-reconstruction assessment of renal allograft vascular integrity, and blood supply analysis of the ureter can be vividly depicted by using Y6CT-NPs with high signal-to-noise ratios upon clinical laparoscopic LED white-light activation. Our work provides efficient molecular design guidelines towards white-light activatable imaging agent and highlights an opportunity for precision imaging theranostics.
虽然第二代近红外二区（NIR-II）荧光成像是实时监测手术操作的一种很有前途的工具，但之前报道的用于体内成像的有机 NIR-II 发光材料主要是通过昂贵的激光或 X 射线激活的，其功率高且照明均匀性差，这极大地限制了它们的临床应用。在这里，我们通过利用纳米粒子中 Y6CT 分子的强分子内/分子间 D-A 相互作用，报告了一种可被白光激活的 NIR-II 有机成像剂，其亮度高达 13315.1，是迄今为止报道的最亮的激光激活 NIR-II 有机对比剂的两倍多。在白光激活下，Y6CT-NPs 不仅可以实现肝缺血再灌注的体内成像，还可以实时监测肾移植手术。在手术过程中，通过使用 Y6CT-NPs，在临床腹腔镜 LED 白光激活下，可获得高信噪比，从而清晰地描绘出肾脏血管、肾移植后血管完整性的重建评估以及输尿管的血液供应分析。我们的工作为高效的分子设计提供了指导方针，朝着白光激活成像剂的方向发展，并为精准成像治疗学提供了机会。
