Duerden B I, Moyes A
J Clin Pathol. 1976 Apr;29(4):286-91. doi: 10.1136/jcp.29.4.286.
Three methods of semiquantitative culture and two techniques of microscopy were compared with a surface viable count for the detection of significant bacteriuria in one thousand midstream specimens of urine. The results obtained with the blotting-paper-strip method on MacConkey agar and with Uricult dip-slides correlated well with the results of the surface viable count, and both methods were suitable for routine use. The blotting-paper-strip method was preferred for laboratory use because of expense but dip-slides are useful for general practice and outpatient clinics. Semi-quantitative culture by Microstix dip-strips gave less accurate results, and the nitrite test area detected only a small proportion of infected specimens. The microscopic examination of a Gram-stained film of the centrifuged deposit of urine specimens yielded more useful information and was more reproducible than examination of a wet film of the untreated urine.
为检测1000份中段尿标本中的显著菌尿，将三种半定量培养方法和两种显微镜检查技术与表面活菌计数法进行了比较。在麦康凯琼脂上使用试纸条法和使用Uricult浸片法获得的结果与表面活菌计数结果相关性良好，这两种方法均适用于常规使用。由于成本原因，试纸条法更适合实验室使用，但浸片法对全科医疗和门诊诊所很有用。Microstix浸条半定量培养结果准确性较低，亚硝酸盐检测区仅检测出一小部分感染标本。对尿标本离心沉淀物的革兰氏染色涂片进行显微镜检查比检查未经处理尿液的湿涂片能提供更多有用信息，且更具可重复性。
