Institute of Physiology, Komi Science Centre, Ural Branch of the Russian Academy of Sciences, FRC Komi SC UB RAS, 50 Pervomayskaya Str., 167982 Syktyvkar, Russia.
Cells. 2024 Jun 27;13(13):1110. doi: 10.3390/cells13131110.
The aim of this study was to investigate the relationships between levels of n-3 essential polyunsaturated fatty acids (n-3 PUFAs) and stable nitric oxide (NO) metabolites in the plasma of athletes.
Highly trained cross-country skiers (males, = 39) were examined. The fatty acid profile of the total plasma lipids was determined by gas chromatography. The plasma NO level was studied by a colorimetric method via reaction with Griess reagent.
A widespread deficiency of essential n-3 PUFAs in the plasma of athletes (more than 80% of the subjects) was demonstrated in association with an imbalance in the levels of nitrates (NO) and nitrites (NO). A lower value of n-3 linolenic acid in the plasma (0.21 mol/%) was associated with a NO level below the normal range (n-3 C18:3 and NO Rs = 0.461; = 0.003). Higher levels of n-3 eicosapentaenoic acid (0.8 mol/%) were associated with a concentration of NO above the normal value (n-3 C20:5 and NO Rs = 0.449; = 0.004).
For the first time, the participation of essential n-3 PUFAs in the nitrite-nitrate pathway of NO synthesis in highly trained skiers was demonstrated.
本研究旨在探讨运动员血浆中 n-3 必需多不饱和脂肪酸（n-3 PUFAs）水平与稳定的一氧化氮（NO）代谢物之间的关系。
对 39 名经过高度训练的越野滑雪运动员进行了检查。通过气相色谱法确定总血浆脂质的脂肪酸谱。通过与格里斯试剂反应的比色法研究血浆 NO 水平。
在运动员的血浆中普遍缺乏必需的 n-3 PUFAs（超过 80%的受试者），同时硝酸盐（NO）和亚硝酸盐（NO）水平失衡。血浆中 n-3 亚麻酸的较低值（0.21 mol%）与低于正常范围的 NO 水平相关（n-3 C18:3 和 NO Rs = 0.461； = 0.003）。较高水平的 n-3 二十碳五烯酸（0.8 mol%）与高于正常值的 NO 浓度相关（n-3 C20:5 和 NO Rs = 0.449； = 0.004）。
首次证明了必需的 n-3 PUFAs 参与了高度训练的滑雪运动员中 NO 合成的亚硝酸盐-硝酸盐途径。
