Department of Biological and Agricultural Engineering, University of California , Davis, One Shields Avenue, Davis, CA, 95616, USA.
Department of Plant Pathology, University of California , Davis, One Shields Avenue, Davis, CA, 95616, USA.
Bioprocess Biosyst Eng. 2024 Oct;47(10):1683-1690. doi: 10.1007/s00449-024-03061-w. Epub 2024 Jul 12.
This study investigated cellobionate production from a lignocellulosic substrate using Neurospora crassa HL10. Utilizing NaOH-pretreated wheat straw as the substrate obviated the need for an exogenous redox mediator addition, as lignin contained in the pretreated wheat served as a natural mediator. The low laccase production by N. crassa HL10 on pretreated wheat straw caused slow cellobionate production, and exogenous laccase addition accelerated the process. Cycloheximide induced substantial laccase production in N. crassa HL10, enabling the strain to yield approximately 57 mM cellobionate from pretreated wheat straw (equivalent to 20 g/L cellulose), shortening the conversion time from 8 to 6 days. About 92% of the cellulose contained in the pretreated wheat straw is converted to cellobionate. In contrast to existing methods requiring pure cellobiose or cellulase enzymes, this process efficiently converts a low-cost feedstock into cellobionate at a high yield without enzyme or redox mediator supplementation.
本研究利用粗糙脉孢菌 HL10 从木质纤维素基质中生产纤维二糖酸盐。利用 NaOH 预处理的小麦秸秆作为基质，避免了添加外源氧化还原介体的需要，因为预处理小麦中的木质素充当了天然介体。由于粗糙脉孢菌 HL10 在预处理的小麦秸秆上产生的低漆酶量导致纤维二糖酸盐的产生缓慢，而外源漆酶的添加加速了这一过程。环己酰亚胺诱导粗糙脉孢菌 HL10 产生大量漆酶，使该菌株能够从预处理的小麦秸秆中产生约 57 mM 的纤维二糖酸盐（相当于 20 g/L 纤维素），将转化时间从 8 天缩短至 6 天。预处理的小麦秸秆中约 92%的纤维素转化为纤维二糖酸盐。与需要纯纤维二糖或纤维素酶的现有方法相比，该过程无需酶或氧化还原介体的补充，就能以高效率、高产量地将低成本原料高效转化为纤维二糖酸盐。
