College of Life Science, Yantai University, Yantai 264005, China.
Molecules. 2024 Jun 24;29(13):2999. doi: 10.3390/molecules29132999.
In our search for a biocompatible composite hemostatic dressing, we focused on the design of a novel biomaterial composed of two natural biological components, collagen and sodium alginate (SA), cross-linked using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) and oxidized sodium alginate (OSA). We conducted a series of tests to evaluate the physicochemical properties, acute systemic toxicity, skin irritation, intradermal reaction, sensitization, cytotoxicity, and in vivo femoral artery hemorrhage model. The results demonstrated the excellent biocompatibility of the collagen/sodium alginate (C/SA)-based dressings before and after crosslinking. Specifically, the femoral artery hemorrhage model revealed a significantly shortened hemostasis time of 132.5 ± 12.82 s for the EDC/NHS cross-linked dressings compared to the gauze in the blank group (hemostasis time of 251.43 ± 10.69 s). These findings indicated that C/SA-based dressings exhibited both good biocompatibility and a significant hemostatic effect, making them suitable for biomedical applications.
在寻找一种具有生物相容性的复合止血敷料时，我们专注于设计一种由两种天然生物成分（胶原蛋白和海藻酸钠）组成的新型生物材料，使用 1-乙基-3-(3-二甲基氨基丙基)碳二亚胺/N-羟基琥珀酰亚胺（EDC/NHS）和氧化海藻酸钠（OSA）交联。我们进行了一系列测试来评估物理化学性质、急性全身毒性、皮肤刺激、皮内反应、致敏性、细胞毒性和体内股动脉出血模型。结果表明交联前后的胶原蛋白/海藻酸钠（C/SA）基敷料具有优异的生物相容性。具体来说，EDC/NHS 交联敷料的股动脉出血模型止血时间明显缩短，为 132.5±12.82s，而空白组中纱布的止血时间为 251.43±10.69s。这些发现表明 C/SA 基敷料具有良好的生物相容性和显著的止血效果，适用于生物医学应用。
