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INTRODUCTION

, derived from the dried leaves of Fort, has been used for centuries as a traditional Chinese herb with antibacterial and antiviral properties. However, both the cultivation conditions and the growth status of Fort have been negatively affected by climatic and environmental degradation, which has made it challenging to accurately assess the quality of . The current quality control system for lacks precision and comprehensive identification indices, and importantly, the cultivation process has not been integrated into this system.

METHODS

In this study, we proposed a novel method to distinguish between different stress subtypes in Fort based on biophoton emission and attempted to explore the potential relationship between the biophoton characteristics of fresh Fort leaves and the quality of . The delayed luminescence (DL) and spontaneous photon emission (SPE) characteristics of fresh Fort leaves under different stress conditions were detected using a biophoton detection system. An attempt was made to differentiate samples subjected to various stress treatments using biophoton characteristic parameters. Additionally, the content of active ingredients was determined by ultra-high performance liquid chromatography, and the inhibitory activity against Escherichia coli and Staphylococcus aureus was evaluated to identify the quality of . Several physiological indicators of fresh Fort leaves, including the photosynthetic pigment content, relative electrical conductivity, and reactive oxygen species production rate were also determined.

RESULT

The differences in physiological indices, active ingredient content, and inhibitory activity indicated that the stress conditions significantly inhibited the growth status of Fort leaves and the herbal quality. Meanwhile, biophoton characteristic parameters were obtained that could accurately and efficiently distinguish fresh Fort leaves between different stress subtypes: initial intensity of DL and counts per second of SPE. Both characteristic parameters were highly correlated with the physiological indicators and quality of .

DISCUSSION

This study has preliminarily demonstrated the feasibility of utilizing biophoton detection technology for the quality evaluation of during cultivation for the first time and provided an improved method for distinguishing samples of various qualities.

INTRODUCTION

METHODS

RESULT

DISCUSSION

引言

来源于Fort的干燥叶，几个世纪以来一直作为具有抗菌和抗病毒特性的传统中草药使用。然而，Fort的种植条件和生长状况都受到气候和环境退化的负面影响，这使得准确评估Fort的质量具有挑战性。目前Fort的质量控制系统缺乏精度和全面的鉴别指标，重要的是，种植过程尚未纳入该系统。

方法

在本研究中，我们提出了一种基于生物光子发射区分Fort不同胁迫亚型的新方法，并试图探索新鲜Fort叶的生物光子特征与Fort质量之间的潜在关系。使用生物光子检测系统检测不同胁迫条件下新鲜Fort叶的延迟发光（DL）和自发光子发射（SPE）特征。尝试使用生物光子特征参数区分经过各种胁迫处理的样品。此外，通过超高效液相色谱法测定活性成分的含量，并评估对大肠杆菌和金黄色葡萄球菌的抑制活性以鉴定Fort的质量。还测定了新鲜Fort叶的几个生理指标，包括光合色素含量、相对电导率和活性氧产生率。